Boric saline solution

The type of eyewash solution recommended is a boric saline solution made up to the following formula ... 

From this material, samples are cut and swelled to constant weight in a buffered saline solution prepared from 8.43 g sodium chloride (NaCl), 9.26 g boric acid (H3BO3), 1.0 g sodium borate (Na3B03), and 0.1 g of the disodium salt of the dihydrate of ethylenediaminetetraacetic acid [Na2 EDTA -(/ 0)21 ini L of distilled water. 

A procedure for immobilization of a P. stutzeri UP-1 strain using sodium alginate was reported [133], This strain does not perform sulfur-specific desulfurization, but degrades DBT via the Kodama pathway. Nevertheless, the report discussed immobilization of the biocatalyst cells in alginate beads with successful biocatalyst recovery and regeneration for a period of 600 h. However, the immobilized biocatalyst did decrease in specific activity, although the extent of loss was not discussed. The biocatalyst was separated after every 100 h of treatment, washed with saline and a boric acid solution and reused in subsequent experiment. The non-immobilized cells were shown to loose activity gradually with complete loss of activity after four repeat runs of 20 hour each. The report does not mention any control runs, which leaves the question of DBT disappearance via adsorption on immobilized beads unanswered and likewise the claim of a better immobilized biocatalyst. 

First, polarograph 5 ml of the buffer solution (v) of boric acid and sodium carbonate, starting from —1.4 V. Then add 0.2, 0.4 and 0.6 ml of serum diluted 1 5 with normal saline solution, and polarograph at a suitable sensitivity (1/1000). Calibrate by adding equal gradated volumes of diluted serum of known protein concentration. 

Ophthalmic Dosage Forms. Ophthalmic preparations can be solutions, eg, eye drops, eyewashes, ointments, or aqueous suspensions (30). They must be sterile and any suspended dmg particles must be of a very fine particle size. Solutions must be particle free and isotonic with tears. Thus, the osmotic pressure must equal that of normal saline (0.9% sodium chloride) solution. Hypotonic solutions are adjusted to be isotonic by addition of calculated amounts of tonicity adjusters, eg, sodium chloride, boric acid, or sodium nitrate. 

Buffer solutions Components of buffer solutions are available from Sigma (St. Louis, MO). Phosphate-buffered saline (PBS) contains 20 mM NaH2P04 and has pH 7.4 (PBS) borate-buffered saline (BBS) contains 20 mM boric acid, pH 8.1. Where indicated, either buffer contained 2 mg/mL bovine serum albumin (PBS-BSA or BBS-BSA). 

Analytical chemistry has found great utility in conductimetric measurements in spite of its apparent nonspecificity. Rapid quantitative accuracy of a few tenths of a percent may be quickly accomplished by direct conductimetric determination of binary electrolytic solutions such as aqueous acids, bases, or salts. A nearly linear increase in conductivity is observed for solutions containing as much as 20% of solute. The concentration of strong solutions, such as the salinity of seawater, may be determined from conductance measurements traces of electrolyte impurities, such as the impurity in ultrapure water, may be reported at the pgl level. Conductimetric titrations may increase the accuracy of endpoint detection and permit titrimet-ric analysis of weak electrolytes, such as boric acid, which is not feasible by potentiometric or colorimetric... 

---