Bone marrow neutrophil

Cramer, E.M., Beesley, J.E., Pulford, K.A.F., Breton-Gorius, J., and Mason, D.Y. (1989) Colocalization of elastase and myeloperoxidase in human blood and bone marrow neutrophils using a monoclonal antibody and immunogold. Am. J. Pathol. 134, 1275-1284. 

Broome, C.S., Whetton, A.D. and Miyan, J.A. (2000) Neuropeptide control of bone marrow neutrophil production is mediated by both direct and indirect effects on CFU-GM. British Journal of Haematology, 108, 140-150. 

Activated leukocytes also express IL-6, which increases the proliferation of antibody-producing lymphocytes (B-lymphocytes) locally and the replenishment of bone marrow neutrophils globally (Fig. 13.8). Another function of IL-6 is to act with IL-1 to increase the hepatic expression and plasma concentration of most blood clotting proteins (Sect. 11.1.1). This stimulation of blood clotting protein synthesis may partially explain the association of periodontitis with an increased likelihood of vascular clotting events, notably heart attacks and strokes (Sect. 13.1.2). 

Bone marrow neutrophil donors 6- to 8-week-old C57/BL6 male or female mice or desired genetically modified mice, such as CCR1-, CXCR2-, C5aR-, or BLTl-deficient mice (Chou et al., 2010 Jacobs et al., 2010 Kim et al., 2006 Sadik et al., 2012). 

Dissect four bones (femurs tibias) from bone marrow neutrophil donor mice, put in 50 ml tubes containing sterile PBS, and place on ice. [Note One mouse (four bones femurs tibias) should yield over 50 million BM cells. If more BM cells from one mouse are desired, two additional bones (humerus) may be also used.]... 

Hematologic Lithium increases bone marrow neutrophil production, protects bone marrow hemopoietic stem cells after exposure to anticancer drugs or radiation, and increases platelet count [71 ]. These effects suggest several potential uses in medicine, such as concomitant use in patients who have clozapine-induced neutropenia. 

Hematopoiesis is defined as the development and maturation of blood cells and their precursors. In utero, hematopoiesis may occur in the liver, spleen, and bone marrow. However, after birth, it occurs exclusively in the bone marrow. All blood cells are generated from a common hematopoietic precursor, or stem cell. These stem cells are self-renewing and pluripotent and thus are able to commit to any one of the different lines of maturation that give rise to platelet-producing megakaryocytes, lymphoid, erythroid, and myeloid cells. The myeloid cell line produces monocytes, basophils, neutrophils, and eosinophils, whereas the lymphoid stem cell differentiates to form circulating B and T lymphocytes. In contrast to the ordered development of normal cells, the development of leukemia seems to represent an arrest in differentiation at an early phase in the continuum of stem cell to mature cell.1... 

WBC differential (bone marrow) Polymorphonuclear neutrophils (PMNs) 3-11 %... 

Martin C, Burdon PC, Bridger G, Gutierrez-Ramos JC, Williams TJ, Rankin SM. Chemokines acting via CXCR2 and CXCR4 control the release of neutrophils from the bone marrow and their return following senescence. Immunity 2003 19 583-593. 

Leukine (sargramostim, GM-CSF) Autologous bone marrow transplantation Neutrophil recovery after bone marrow transplantation Berlex Labs... 

The G-CSF receptor has been well characterized. It is a single transmembrane polypeptide found on the surface of neutrophils, as well as in various haemopoietic precursor cells, platelets, endothelial cells and, notably, various myeloid leukaemias. (Myeloid means derived from bone marrow leukaemia refers to a cancerous condition in which there is uncontrolled overproduction of white blood cells in the bone marrow or other blood-forming organs. The white cells produced are generally immature/abnormal and result in the suppression of production of healthy white blood cells.)... 

G-CSF and GM-CSF have also found application after allogenic or autologous bone marrow transplantation, to accelerate neutrophil recovery. (Allogenic means that donor and recipient are different individuals, and autologous means that donor and recipient are the same.)... 

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