Bone marrow neutrophil isolation

There has been very little experimental work conducted with natural products from the phylum Bryozoa. The family of bryostatins, macrolide polyketides isolated by Pettit from the bryozoan Bugula neritina, is certainly of greatest significance in terms of biomedical potential [92]. Bryostatin 1 (57) exhibits selective activity against B-cell lymphomas and leukemias, [93] and directly stimulates bone marrow progenitor cells to form colonies that functionally activate neutrophils [94]. Additionally, bryostatin 1 activates protein kinase C [95] and has immunomodulatory activity both in vitro and in vivo [96]. In combination with the vinca alkaloid vincristine, bryostatin 1 inhibits the growth of lymphoma cells without adverse effects on bone marrow cells [97]. 

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