Bone extracellular matrix

Cell differentiation studies are used to follow the development of cell phenotype by analyzing the concentration of two proteins directly related to bone extracellular matrix mineralization alkaline phosphatase and the osteocalcin. Cell differentiation studies performed on the G5 glass have shown that it induces an earher differentiation of the osteoblastic cells than the polysterene plate controls (unpublished data) (see Fig. 2). Consequently, a faster bone formation could be obtained. 

Araujo, J.V., Martins, A., Leonor, I.B., Pinho, E.D., Reis, R.L. and Neves, N.M. 2008. Surface controlled biomimetic coating of polycaprolactone nanofiber meshes to be used as bone extracellular matrix analogues. 

Datta, N., Holtorf, H.L., Sikavitsas, V.L, Jansen, J.A., Mikos, A.G., 2005. Effect of bone extracellular matrix S5uithesized in vitro on the osteoblastic differentiation of marrow stromal cells. Biomaterials 26, 971-977. 

Sawkins, M.J., Bowen, W., Dhadda, P, Markides, H., Sidney, L.E., Taylor, A.J., Rose, F.R., Badylak, S.F., Shakesheff, K.M., White, L.J., 2013. Hydrogels derived from demineralized and decellularized bone extracellular matrix. Acta Biomater. 9, 7865-7873. 

Owen, T. A., Aronow, M., Shalhaub, V., Barone, L. M., Wilming, L., Tassinari, M. S., Kennedy, M. B., Pockwinse, S., Lian, J. B., and Stein, G. S., 1990, Progressive development of the rat osteoblast phenotype in vitro Reciprocal relationship in expression of genes associated with osteoblast proliferation and differentiation during the formation of the bone extracellular matrix, J. Cell Physiol 143 420-430. 

To mimic the constituents of natural bone extracellular matrix, the crosstinked fibers were coated with carbonated hydroxyapatite through a biomimetic precipitation, resulting in an attractive bio-... 

Gentili, C., Cancedda, R., 2009. Cartilage and bone extracellular matrix. Current Pharmaceutical Design 15, 1334-1348. 

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