Cross-linked agarose

Support materials for low-pressure affinity chromatography include agarose (cross-linked with epichlorohydrin), cellulose, dextran, silica, and polyacrylamide 62 in HPLC a rigid, highly porous, hydrophilic polymer is typically used. Large pore sizes are necessary, as either the analyte or the affinity ligand are macromolecules. To provide unhindered access of... 

MANAE-Agarose Cross-linked with glutaraldehyde One gram of the YLL immobilized onto MANAE agarose was incubated with 1% (v/v) glutaraldehyde solution in sodium phosphate buffer 5 mM pH 7 and 25°C for 1 h, under mild stirring. After that, the suspension was washed with 5 mM phosphate buffer and then filtered and stored at 4°C. The samples of the suspensions were withdrawn, and YLL activity was measured using /)NPB as substrate as described above. 

Agarose, cross-linked with epichlorohydrin to render the beads sturdier and more heat-resistant, has been activated with cyanogen bromide and coupled with glycine methyl ester." The ester was then converted into the glycyl... 

Agarose cross-linked with 2,3-dibromopropanol Sepharose CL DMF/0.1 MLiCl Kraft Ugnin [1]... 

Highly cross-linked 6% agarose Cross-linked 6% agarose... 

Agarose cross-linked with epichlorohydrin reacted with 1-bromohexane to give a derivative that adsorbed p-amylase in an active form. " Hexyl- and octyl-substituted, epichlorohydrin-cross-linked agaroses containing mercapto-groups on the side-chains have been used for the reversible immobilization of P-amylase and glucoamylase. ... 

Active immobilized forms of j3-amylase have been prepared by the reaction of the enzyme with agarose cyclic imidocarbonate and its derivatives, and by hydrophobic interaction with alkyl derivatives of agarose cross-linked with epichlorohydrin. ... 

Macroporous agarose cross-linked with epichlorohydrin has been reduced and then activated with cyanogen bromide to introduce cyclic imidocarbonate groups this material reacted with the 3- and 4-isomers of A-(6-aminohexanoyl)-1-aminophenyltrimethylammonium bromide hydrobromide to give affinants that were used in the purification of acetylcholinesterase. ... 

Blood-group A substance has been partially deacetylated with toluene-p-sulphonic acid. The deacetylated product reacted with a cyclic imidocarbonate derivative of agarose cross-linked with 2,3-dibromopropanol to give a material used as an immunoadsorbent for anti-(blood-group A) antibodies. 

SEC, known as gel-filtration chromatography, is a chromatographic method in which molecules are separated according to their sizes and molecular weights [15] and can be used as a primary fractionation method for large amounts of crude extract. It involves the use of porous gel molecules of agarose, cross-linked dextran, or polymers of acrylamide that allow the separation of compounds based on their molecular sizes. There is no sample loss because generally solutes used do not interact with the stationary phase, an... 

An example of a size-exclusion chromatogram is given in Figure 7 for both a bench-scale (23.5 mL column) separation and a large-scale (86,000 mL column) mn. The stationary phase is Sepharose CL-6B, a cross-linked agarose with a nominal molecular weight range of 5000-2 x 10 (see Fig. 6) (31). 

Superdex is a composite media of agarose and dextran. The base particle is formed of cross-linked agarose to which dextran is then covalently attached. The resulting family of Superdex-based gels has been constructed to provide... 

Superose gel material of Pharmacia Biotech is a highly epichloro-hydrine cross-linked agarose matrix that has a pH range of 3-12 (short term 1-14). Hydrophilic interactions may be noticeable for lipids, peptides, and small aromatic compounds, but such interactions might even improve resolution. Superose medium is available in two different porosities Superose 6 HR 10/ 30 (bead size 13 2 /um maximum pressure 1.5 MPa) and Superose 12 HR 10/30 (bead size 10 2 /um maximum pressure 3.0 MPa). 

Sepharose CL gels of Pharmacia Biotech are cross-linked agarose derivatives with cross-links formed by 2,3-dibromopropanol. Compared to pure agarose, the cross-linking improves chemical and physical resistance of the... 

TABLE 16.5 Producers (Pharmacia Biotech) Specification of Fractionation Ranges of Epichloro-Hydrine Cross-Linked Agarose-Based Superose Geis... 

Prepacked columns with cross-linked high-resolution (HR) agarose gels provide a high number of theoretical plates and fast separations (29,30).The Superose gel material of Pharmacia Biotech is a highly epichloro-hydrine cross-linked... 

B. Type 3 physical cross-linked gels, agarose... 

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