Blood mononuclear cells, binding

Bryostatin 1 binds to the regulatory domain of protein kinase C short-term exposure promotes activation of protein kinase C, whereas prolonged exposure promotes significant down-regulation (4). In preclinical and phase I clinical studies it had promising antitumor and immuno-modulating effects. It amplifies expansion of myeloid and erythroid progenitor cells stimulated by the cytokines GM-CSF, M-CSF, and IL-3. Similarly, it induces the production of peripheral blood mononuclear cells with enhanced lymphokine-activated killer cell activity and proliferation in the presence of IL-2 (5). 

Rocca, P., Bellone, G., Benna, P., Bergamasco, B., Ravizza, L., and Ferrero, P., Peripheral-type benzodiazepine receptors and diazepam binding inhibitor-like immunoreactivity distribution in human peripheral blood mononuclear cells, Immunopharmacology, 25, 163, 1993. 

An IL-1 receptor antagonist (IRAP) has been isolated and purified from activated monocytes. IRAP inhibits IL-1 bio activity by binding competitively at the IL-1 receptor (Eisenberg et al. 1990, Hannum et al. 1990, Mazzei et al. 1990). In vitro IRAP can attenuate IL-1-dependent fibroblast proliferation, collagenase synthesis, and prostaglandin E2 release (Hannum et al. 1990). IRAP suppressed IL-l-induced monocyte production of IL-1, tumour necrosis factor-a, or interleukin 6. Multiple hpid oxidation products in low density lipoproteins induce IL-ip release from human blood mononuclear cells (Thomas et al. 1994). 

Zhao, L., J. Yin, D. Li, C. Lai, X. Chen and D. Ma, 2005. Conjugated linoleic acid can prevent tumor necrosis factor gene expression by inhibiting nuclear factor binding activity in peripheral blood mononuclear cells from weaned pigs challenged with lipopolysaccharide. Arch. Anim. Nulr. 59, 429-438. 

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