Blood groups H determinant

SOLID-PHASE SYNTHESIS OF THE BLOOD GROUP H DETERMINANT 23... 

Ernst, L. K., Rajan, V. P., Larsen, R. D., Ruff, M. M. and Lowe, J. B. (1989). Stable expression of blood group H determinants and GDP-L-fucose 0-D-galactoside 2-a-L-fucosyltransferase in mouse cells after transfection with human DNA. J. Biol. Chem. 264, 3436-3447. 

The Chemical Synthesis of 2-Acetamido-2-deoxy-4-(3-(a-L-fucopyra-nosyl)-3-<9-(/l-D-galactopyranosyl)-D-glucose. The Lewis a Blood-Group Antigenic Determinant, R. U. Lemieux and H. Driguez, J. Am. Chem. Soc., 97 (1975) 4063 1069. 

Synthesis of Type 2 Human Blood Group Antigenic Determinants. The H, X and Y Haptens and Variations of the H Type 2 Determinants as Probes for the Combining Site of the Lectin 1 of Ulex europaeus, O. Hindsgaul, T. Norberg, J. LePendu, and R. U. Lemieux, Carbohydr. Res., 109 (1982) 109-142. 

The immunochemical studies of Kabat and coworkers200 686 revealed some striking subtleties in Lotus tetragonolobus sugar-specificity. Precipitin reactions between the lectin and several blood-group H substances demonstrated strong affinity of the lectin for this determinant. H substances precipitated 83-88% of the purified Lotus lectin at... 

Fuc(al-4) were also observed in A. maculatum (Fig. 4). Other unusual sequences, namely the dimer H epitope Fuc(al-2)Gal(Pl-3)[Fuc(al—2)]Gal, and blood group A determinant terminated with an additional a-l,3-linked GlcNAc unit, were found in X. laevis (Fig. 5). 

The GalNAcal-3 residue of blood group A is added to the H determinant by blood group A a3-GalNAc-transferase (EC 2.4.1.40), and the blood group B determinant similarly by blood group B a3-Gal-transferase (EC 2.4.1.37). A related linear B o 3-Gal-transferase (EC 2.4.1.151) from nonprimates synthesizes the linear B determinant on terminal Gal residues without the Fucal-2 substitution. These three enzymes are retaining transferases of the CAZy GT6 family with a GT-A fold (Table 4). 

C26H45N0,9 675.637 Serologically active determinant isolated by alkaline degradation of the human blood group H, Le glycoprotein. 

The ABO substances have been isolated and their structures determined simphfied versions, showing only their nonreducing ends, are presented in Figure 52—6. It is important to first appreciate the structure of the H substance, since it is the precursor of both the A and B substances and is the blood group substance found in persons of type O. H substance itself is formed by the action of a fiicosyltransferase, which catalyzes the addition of the terminal fiicose in al —> 2 linkage onto the terminal Gal residue of its precursor ... 

Scheme 2.9 Solid-phase synthesis of an H-type 2 blood group determinant.

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