Black plate

Scbwarz-blecb, n. black plate (untinned iron plate), -blei, n. black lead (graphite), -blelerz, n. black lead spar (carboniferous cerussite). 

Although other assay containers can be used (e.g., coated tubes or beads), the most popular one is the 96-well microtiter plate. ELISAs that are detected in a microplate luminometer must be performed in opaque white or black plates. 

Plate cells at the appropriate density in a 96-well white/black plate. Seed 4 wells for each clone (see Note 12). 

Streptonegrin (negrin, 5-amino-6-[7-amino-5,8-dihydro-6-methoxy-5,8-dioxo-2-quinolinyl]-4-[2-hydroxy-3,4-dimethoxyphenyl]-3-methyl-2-pyridinecarboxylic acid) [3930-19-6] M 506.5, m 262-263°, 275°(dec). Purified by TLC on pH 7-buffered silica gel (made from a slurry of Silica Gel 60 and 4(X)ml of 0.05M phosphate buffer pH 7.0) and eluted with 5% MeOH/CHClj. The extracted band can then be recrystd from Me2CO or dioxane as almost black plates or needles. It is soluble in pyridine, Me2NCHO, aqueous NaHCOy (some dec), and slightly soluble in MeOH, EtOH, EtOAc and H2O. It has a pKa value in the range 6.2-6.4 (dioxane/H2O 1 1) and UV 248, 375-380nm (e 38400 and 17400). [Weinreb et al. JACS 104 536 1982 Rao et al. JACS 85 2532 1963]. It is an antineoplastic and causes severe bone marrow depression [Wilson et al. Antibiot Chemother 11 147 7967]. 

The black plates which are formed along with LiPt(mnt) in the add oxidation of H2[Pt S2C2(CN)2 2]/LiCl mixtures in acetone-water have been shown to be... 

For luciferase assays, use white 384-well tissue culture treated assay plates (Costar, Acton, MA). For imaging assays, use black plates with clear bottoms. Either plastic-bottomed (cat. no. 3712, Costar) or glass-bottomed (MGB 101-12, Matrical, Spokane, WA) plates can be used. 

The energy transfer per unit time and area by radiation between two infinite parallel black plates is... 

Two infinite black plates at 800 and 300°C exchange heat by radiation. Calculate the heat transfer per unit area. 

Two infinite black plates at 500 and 100°C exchange heat by radiation. Calculate the heat-transfer rate per unit area. If another perfectly black plate is placed between the 500 and I00°C plates, by how much is the heat transfer reduced What is the temperature of the center plate ... 

Two parallel black plates 0.5 by 1.0 m are spaced 0.5 m apart. One plate is maintained at 1000°C and the other at 500°C. What is the net radiant heat exchange between the two plates ... 

Two parallel black plates are separated by a distance of 0.7 m and maintained at temperatures of 200 and 500°C. Between the planes is a gas mixture of 20 percent C02, IS percent water vapor, and 65 percent N2 by volume at a total pressure of 3 atm. The gas temperature is 1000°C. Calculate the heat exchange with each plate. 

This is an interesting example of combined radiation and convection heat-transfer analysis. We designate the black plate as surface 1, the glass as surface 2. and the surroundings as surface 3. We assume no absorption of the solar energy in the glass. For the black plate... 

The selection of the type of assay plate is also dependent on the type of signal(s) to be detected and whether a clear bottom is required to observe cells under a microscope. Opaque black plates are often chosen for fluorescent assays and white plates are best for luminescent assays because of... 

Addition of sulfide ions to aqueous solutions of Ni2+ precipitates black NiS. This is initially freely soluble in acid, but like CoS, on exposure to air it soon becomes insoluble owing to conversion to Ni(OH)S. Fusion of Ni, S, and BaS gives BaNiS2, which forms black plates this product is metallic and has Ni in square pyramidal coordination. 

Molybdenum. The principal ore of molybdenum is molybdenite, MoSg, which occurs especially in a great deposit near Climax, Colorado. This mineral forms shiny black plates, closely similar in appearance to graphite. 

Silver Dichromate, Ag2Cr207, is prepared by the addition of silver nitrate to a hot solution of potassium dichromate containing a large excess of nitric acid, and cooling till crystallisation occurs. The salt may readily be recrystallised from nitric acid, traces of which are, however, retained. It crystallises as red-black plates in the triclinic system, and has a density of 4-770. It cannot be dried without decomposition, for it is readily decomposed by water with formation of the neutral chromate and chromic acid. ... 

A gas at 1200 K and 1 aim consists of 10 percent COj, 10 percenl H2O, 10 percent Oj, and 70 percent Nj by volume. The gas flows betu een Iwo large parallel black plates main-lained at 600 K. If the plates are 20 cm apart, dclennine the lale of heat transfer from the gas lo each plate per unit surface area. 

The light reflected from white and black plates is monitored in every measurement ... 

The black plate is used to compensate for a variation in the quantity of inner stray light of the optical measurement unit (the inner stray light means the light which is detected at the measurement of a completely black plate without invading light into the optical measurement unit from the outside). 

After the black plate is run it is left to dry for about two hours. Then it is turned over... 

The azulene (156) slowly condenses with (7a) to the red-violet azulene diene-amine (157) and cyclization occurs at 150-200°, giving in 58% yield by a 70% conversion a mixture of the expected l,2-dihydrodicyclopenta(e,f-k,l)heptalene (158) and the azupyrene (159), formed by simultaneous dehydrogenation74. Treatment of the mixture with chloranil in benzene leads quantitatively to the pure (159), bronze-shining, black plates, mp. 257-259° (subl.). 

Properties Heavy, grayish-black plates or granules having a metallic luster characteristic odor. Readily sublimed having a violet vapor. D 4.98, mp 113.5C, bp 184C. Soluble in alcohol, carbon disulfide, chloroform, ether, carbon tetrachloride, glycerol, and alkaline iodide solutions insoluble in water. A semiconductor. 

This approach is illustrated on the example of LYP PTPN22), a potential drug target in autoimmunity [30-32]. This assay was developed in 384-well black plates. Results are published at PubChem (http //pubchem.ncbi.nlm.nih.gov). Assay ID 640. 

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