Bioprocess Developments

Next, some of the dedsions involved in bioprocess development will be considered. 

Developing a bioprocess for removing heteroatoms from petroleum or for upgrading heavy crudes involves two main components. The first is biocatalyst development and second is bioprocess development. Both of these topics are discussed in this book,... 

Abstract This chapter embodies two sections. In the first section a survey of the state of the art of azo-dye conversion by means of bacteria is presented, with a focus on reactor design and operational issues. The relevance of thorough characterization of reaction kinetics and yields is discussed. The second section is focused on recent results regarding the conversion of an azo-dye by means of bacterial biofilm in an internal loop airlift reactor. Experimental results are analyzed in the light of a comprehensive reactor model. Key issues, research needs and priorities regarding bioprocess development for azo-dye conversion are discussed. 

Murhammer and Goochee [93, 94] have confirmed the beneficial effect of Pluronic F-68 addition to insect cell cultures and its protective effect upon bubble damage. The use of PF-68 is today ubiquitous in bioprocess development involving insect cell culture technology, although it may conceivably interfere with VLP formation in some instances. For a more detailed study of the effect of bubble in cell damage mechanisms and the mechanism of protection of PF-68 see the review by Chalmers and the references cited therein [95]. 

By way of genetic engineering, microorganisms with meiabolic capacities, unknown of as recently as 15 years ago. have been produced. Coal bioprocessing developments are directed to three objectives ... 

Stockar U von, Marison IW, Birou B (1988) 1st Swiss-Japanese joint meeting on bioprocess development, Interlaken, Switzerland... 

Integration of Systems Biology with Bioprocess Development for L-Threonine Production... 

Table 2 Recent key contributions of 13C metabolic flux analysis to Corymbacterium glutamicum. The compilation of studies is partly adapted from an actual review on metabolic flux analysis in bioprocess development [87]...

Deckwer W-D, Jahn D, Hempel D, Zeng A-D (2006) Systems biology approaches to bioprocess development. Eng Life Sci 6 455 -69... 

Miniaturization in biocatalysis and fermentation is another necessary step. This will allow continuous processes with the benefits that could derive in terms of process intensification and reduction of waste. Miniature (less than 10 mL) stirred reactors and microtiter plates (MTP) have been introduced mainly with the idea of allowing high-throughput screening to speed up bioprocess development, even though they are available now also for production uses [172-174]. Notably, problems emerge with these miniature bioreactors (MBRs), such as evaporation and surface tension, which determine the performances, but which are masked in larger bioreactors. 

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