Biomol Green

Biomol Green reagent (Biomol International, Plymouth Meeting, PA, USA). 

Stop reaction with 100 pL of Biomol Green reagent and measure absorbance at 620 nm. 

HTS Protocol for Using pNPP and Free Phosphate Detection with the Biomol Green Reagent... 

Important This assay is not compatible with buffers containing carboxylic add groups, such as sodiiun dtrate or 2i7-(2-acetamido)-iminoacetic acid (ADA), because the Biomol Green complex formation is compromised in such buffers. 

Branchini, B. R., Lusins, J. O., and Zimmer, M. (1997). A molecular mechanics and database analysis of the structural preorganization and activation of the chromophore-containing hexapeptide fragment in green fluorescent protein. /. Biomol. Struct. Dyn. 14 441-448. 

Ema, T., Okita, N., Ide, S. and Sakai, T., Highly enantioselective and efficient synthesis of methyl (R)-o-chloromandelate with recombinant E. coli toward practical and green access to clopidogrel. Org. Biomol. Chem., 2007, 5, 1175-1176. 

Grable, J., Frederick, C. A., Samudzi, C., Jen-Jacobsen, L., Lesser, D., Greene, P, Boyer, H. W, Itakura, K. and Rosenberg, J. M. (1984). Two-fold symmetry of crystalhne DNA-EcoRl endonuclease recognition complexes. /. Biomol. Struct. Dynam. 1,1149-1160. 

Stokes, D.L. Green, N.M. (2003) Structure and function of the calcium pump. Annu. Rev. Biophys. Biomol. Struct. 32, 445-468. Advanced review. 

Pfleger N, Lorch M, Woemer AC et al (2008) Characterisation of Schiff base and chromophore in green proteorhodopsin by solid-state NMR. J Biomol NMR 40 15-21... 

Alcalde M, Fenera M, Plou FJ et al. (2006) Environmental biocatalysis from remediation with enzymes to novel green processes. TIBTECH 24(6) 281-287 Alexeeva M, Carr R, Turner NJ (2003) Directed evolution of enzymes new biocateilysts for asymmetric synthesis Oig Biomol Chem 1 4133 137 Allen G, Lowe G (1973) Investigation of the active site of papain with fluorescent probes. Biochem J 133 679-686... 

Philip, D., Unni, C., Aromal, S.A., Vidhu, V.K., 2011. Murraya koenigii leaf-assisted rapid green synthesis of silver and gold nanoparticles. Spectrochim. Acta A Mol. Biomol. Spectrosc. 78 (2), 899—904. 

L. Green, Y. Li, T.J. White, A. Urbas, T. Running, Q. Li, Light-driven molecular switches with tetrahedral and axial chirality. Org. Biomol. Chem. 7, 3930-3933 (2009)... 

Angeiici G, Contaldi S, Green SL, Tomasini C (2008) Org Biomol Chem 6 1849... 

Clark JH, Luque R, Matharu A. (2012). Green chemistry, biofuels, and biorefinery, Annu Rev Chem Biomol Eng, 3, 183-207. 

In addition to her job at GSK she is an adjunct professor in Chemical and Biomol-ecular Engineering at North CaroHna State University (NCSU), and serves on the Governing Board of the American Chemistry Society s Green Chemistry Institute. 

M. J. Aliaga, D. J. Ramon, M. Yus, Org. Biomol. Chem. 2010, 8, 43. Impregnated copper on magnetite an efficient and green catalyst for the multicomponent preparation of propargylamines under solvent free conditions. 

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