Biogenic amines molecular determinants

On the other hand, qualitative measurements have also been described to indicate the presence of amines in wine. The potential of biogenic amines to appear in wine can be determined by using molecular tools which detect the presence of decarboxylase-positive microorganisms. 

A wide variety of fluorescent molecular probes have been demonstrated to be suitable for excitation by the He-Cd laser 4-bromomethyl-7-methoxycoumarln has been employed for the detection of carboxylic and phosphoric acids (44,55), 7-chlorocarbonyl-methoxy-4-methylcoumarln for hydroxyl compounds (42), 7-lsothlocya-nato-4-methylcoumarln for amines and amino acids (55), 7-dlazo-4-methyl-coumarln for a variety of aromatic compounds (55), and terbium chelate molecules with long fluorescence lifetimes ( 1 ms) for protein analysis (56). In this study, we examined the utility of l-dlmethylamlnonaphthalene-5-sulfonyl chloride (dansyl chloride) as a sensitive and selective reagent for the determination of biogenic amines and amino acids. 

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