Biogenic amines capillary columns

Song Y, Quan Z, Evans JL, Byrd EA, Liu YM. 2004. Enhancing capillary liquid chromatography/tandem mass spectrometry of biogenic amines by pre-column derivatization with 7-fLuoro-4-nitrobenzoxadiazole. Rapid Commun Mass Spectrom 18 989. 

Oguri, S., Maeda, Y., and Mizusawa, A. (2004). On-column derivatization-capillary electro-chromatography with o-phthalaldehyde/alkylthiol for assay of biogenic amines. /. Chromatogr. A 1044, 271-276. 

A replaceable C disk electrode (two-electrode system) was used for end-column amperometric detection of biogenic amines. The electrode was inserted through a guide tube and was 30 5 [tm away from the capillary exit. This configuration allowed easy replacement of the electrode, especially after biofouling. The electrode was situated at a central position to maximize coulombic... 

Biogenic amines are usually detected by LC with a pre- or postcolumn derivatization with o-phthalaldehyde in the presence of mer-captoethanol, and fluorimetric detection of derivatives. A sample derivatization also has to be done to perform GC/MS analysis of grape juice or wine. Amines are distilled from the alkalized sample and trapped in an acidified solution. After concentration under vacuum, salts of ethylamine, dimethylamine, ethylamine, diethyl-amine, n-propylamine, isobutylamine, a-amylamine, isoamylamine, pyrrolidine, and 2-phenethylamine are derivatized with trifluoroacetic (TEA) anhydride. Their derivatives are extracted with ethyl ether. GC/MS is performed using a capillary fused silica PEG column with an oven temperature programmed for 8 min at 70 °C, l°C/min to 160°C, isotherm for 90min (Daudt and Ough, 1980). 

We have developed two-dimensional CE systems for the characterization of proteins and biogenic amines. The use of this technology for chemical cytometry is similar to the use of onedimensional electrophoresis a cell is aspirated into the column, lysed, and its components labeled with FQ. For two-dimensional electrophoresis, components are separated based on CSE in the first-dimension capillary. Fractions are then transferred across an interface to a second capillary, where they undergo additional separation based on micellar electrokinetic chromatography (MECC) before detection by fluorescence. The voltage drop across the first capillary is set to zero during the second dimension separation, holding components stationary. In a typical experiment, 300 fractions are transferred between capillaries under computer control. 

Figure 432 Fast separation of biogenic amines together with common inorganic cations on lonPac CS19-4 im in the capillary format. Column dimensions 250 mm X 0.4 mm i.d. column temperature 30°C eluent methanesulfonic acid (EG) gradient 9mmol/L linearly to 70mmol/L in 7 min flow rate ...

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