Biochips electrochemical detection

Electrochemical detection is a powerful approach in DNA analysis and a microfluidic-based electrochemical biochip for DNA hybridization assay has been developed recently [23, 24]. Ben-Yoav et al. [24] presented an electrochemical microfluidic biochip for DNA hybridization. An array of gold/platinum electrode was designed and patterned in the biochip. The DNA probes were functionalized on the electrodes and the hybridization events were detected by electrochemical impedance spectroscopy (EIS) analysis. 

In this study, we have demonstrated a new electrochemical method for highly sensitive, accurate, and rapid multiplex detection of colon cancer cells response to differentiation therapy. The development of an electronic biochip array system, adjusted to biological experiments can help tailor cancer treatment to individual patients. Human colon cancer cells, HT-29, were treated with the differentiation therapy drag agents BA, AN-7, and AN-9, and the cells response was simultaneously measured on hue and compared. This microarray technology provides the ability to test on line the affect of multi-drag agents, and to tailor effective therapy to the individual. 

Choi, Y. S. Park, D. H. Electrochemical single nucleotide polymorphism (SNP) detection using a microelectrode array biochip by Hoechst 33258. J. Korean Phys. Soc. 2005, 46, 1445-1451. 

Zhao, C., Wittstock, G. Scanning electrochemical microscopy for detection of biosensor and biochip surfaces with immobilized pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase as enzyme label. Biosens Bioelectron 2005, 20, 1277-1284. 

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