Bioassay directed chemical

Fernandez P, M Grifoll, AM Solanas, JM Bayiona, J Albalges (1992) Bioassay-directed chemical analysis of genotoxic compounds in coastal sediments. Environ Sci Technol 26 817-829. 

Hannigan MP, Cass GR, Penman BW, Crespi CL, Busby JWF, Lafleur AL, Thilly WG, Simoneit BRT (1998) Bioassay-directed chemical analysis of Los Angeles airborne particulate matter using a human ceil mutagenicity assay. Environ Sci Technol 32 3502-3514... 

Analysis of the complex mixtures of gaseous and/or particulate POM in primary emissions or ambient air is a daunting task, given the huge numbers of species and the small concentrations. The development of the Salmonella typhimurium assay has helped to direct such analysis through the technique of bioassay-directed chemical analysis. 

Bioassay-Directed Chemical Analysis for PAHs and PACs in Fine Ambient Aerosols Using a Human Cell Assay... 

As discussed earlier, with respect to bacterial mutagens, one of the applications of mutagen assays is their use in focusing analytical studies directed to determining the chemical composition. This approach was also used by Hannigan and co-workers (1998), who carried out a bioassay-directed chemical analysis using the hlAlv2 human cell line of a composite sample made up of a portion of every 24-h filter sample. 

Hannigan et al. (1998) reported that most of the mutagenicity of the extract of the whole, composite sample of respirable ambient aerosols was in the whole aromatics fraction, 83 vs 17% in the aliphatic portion. Table 10.25 summarizes the results of this bioassay-directed chemical analysis for the aromatics. As seen in Table 10.26, six PAHs, cyclopenta[c[Pg.497]

TABLE 10.25 Mass Concentrations of Individual Genotoxic PAHs and PACs in the Whole Sample and in Subfractionated Extracts,1 Their Absolute Mutagenic Potencies and Potencies Relative to Benzotalpyrene = 1.00, and Contributions of Each to the Overall Total Potency of a Composite Mixture of Ambient Aerosols Collected at Four Urban Sites in Southern California for One Year (1993) and Subjected to Bioassay-Directed Chemical Analysis Using the hlAlv2... 

TABLE 10.26 Concentrations, Potencies, and Percentage Contributions of Selected PAHs and PACs Tested in the hlAlv2 Human Cell Assayb and Positively Identified in the Bioassay-Directed Chemical Analysis of a Composite Whole Sample Made Up of Fine Ambient Aerosols Collected at Four Urban Sites in the Los Angeles Air Basin 1 0... 

Bioassay-Directed Chemical Analysis for Vapor-Phase and Particle-Phase PAHs and PACs in Ambient Air Using Bacterial Assays... 

Hannigan, M. P., G. R. Cass, B. W. Penman, C. L. Crespi, A. L. Lafleur, W. F. Busby, Jr., W. G. Thilly, and B. R. T. Simoneit, Bioassay-Directed Chemical Analysis of Los Angeles Airborne Particulate Matter Using a Human Cell Mutagenicity Assay, Environ. Sci. Technol., 32, 3502-3514 (1998). 

Schuetzle, D and J. Lewtas, Bioassay-Directed Chemical Analysis in Environmental Research, Anal. Chem., 58, 1060A-1075A (1986). 

Zinbo, M., D. Schuetzle, D. P. H. Hsieh, N. Y. Kado, J. M. Dasiey, and L. A. Gundel, An Improved Fractionation Procedure for the Bioassay-Directed Chemical Analysis of Ambient Air Particulate Extracts, Anal. Sci., 8, 461-468 (1992). 

Rastall, A.C., D. Getting, J. Goddard, D.R. Roberts, and L. Erdinger. 2006. A biomimetic approach to the detection and identification of estrogen receptor agonists in surface waters using semipermeable membrane devices (SPMDs) and bioassay-directed chemical analysis. Environ. Sci. Pollut. Res. 13 256-267. 

Casellas, M., Fernandez, P., Bayone, J. M., and Solanas, A. M., Bioassay-directed chemical analysis of genotoxic compounds in urban airborne particulate matter from Barcelona, Chemosphere, 30, 725-740, 1995, Spain. 

We proceeded with the bioassay-directed chemical study of this plant. From the active fractions we isolated bisabolene- and silphinene-type sesquiterpenes (23 and 24). These compounds are strong CPB antifeedants in both choice and no-choice assays, the adult insects being the most sensitive to their action (table 6) [32], Their antifeedant potencies were similar (23) or stronger than that of limonin (24), a CPB antifeedant triterpene [33],... 

During the past three years several procedures have been developed for simplifying the analysis of complex environmental samples. These techniques have included "Bioassay Directed Chemical Analysis," (15) which uses short-term bioassays for determining which chemical fractions should be prioritized for detailed chemical analysis and "Survey Chemical Analysis", which utilizes analytical techniques such as hl h resolution mass... 

Grifoll, M. Solanas, A.M. Bayona, JM. Bioassay-directed chemical characterization of genotoxic agents in the dissolved and particulate water phases of Besos and Llobregat rivers, Barcelona, Spain. Arch. Environ. Contam. Toxicol. 1992, 23 (1), 19-25. 

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