Bioactive factors

CSF-I is thus one major bioactive factor released by MSS cells which can stimulate Myl-D7. However, it should be noted that stimulation of lineage negative Myl-D7 cells by E.coli-derived recombinant-CSF-1 at equivalent CSF-i concentrations was by far less efficient than CM from MSS cells. Furthermore, the stimulation of lineage negative cells by MSS supernatants was far better than that of L929 supernatants having similar concentrations of CSF-I (data not shown). Thus it is likely that MSS stromal cells produce additional factors that are required for Myl-D7 cell maintenance. 

Buts, J. P. (1998). Bioactive factors in milk. Arch. Pediatr. 5, 298-306. 

The structural stability of a material relates to its ability to go through the freeze drying process without change in size, porous structure, and shape. The maximum allowable temperature in the frozen layer is determined by both structural stability and product stability (e.g., product bioactivity) factors that is, the maximum value of the tanperature in the frozen layer during the primary drying stage must be such that the drying process is conducted without loss of product property (e.g., bioactivity) and structural stability. 

Boden SD. Bioactive factors for bone tissue engineering. Clin Orthop Relat Res 1999 367 584-94. 

The key to the successful clinical application for hard tissue repair using the ECM-derived tissues is the preservation of bioactive factors and the elimination of any immunogenic substance remained in the source tissue during the decellularization or demineralized procedures. However, the current acellular methods normally cause loss of bioactive factors and/or the destruction of the ultrastructure due to the extreme conditions experienced during the process (Zimmermann and Moghaddam, 2011 Badylak et al., 2011). Therefore, further optimization of the ECM scaffolds is required in the following studies so as to ensure the safety and effectiveness of these promising scaffolds. 

Bioactive factor release (growth factors, cytokines)... 

Ewaschuk JB, Diaz H, Meddings L, et al. Secreted bioactive factors from Bifidobacterium infantis enhance epithelial cell barrier function. 

The progenitor of neutrophils changes its morphology and function to resemble mature neutrophils that are found in peripheral blood and inflamed tissues under the stimulation of certain bioactive factors (differentiation factors). 

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