Bile acids, high-performance liquid chromatography

Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. Conventionally, instrumental chiral separations have been achieved by gas chromatography and by high performance liquid chromatography.127 In recent years, there has been considerable activity in the separation and characterization of racemic pharmaceuticals by high performance capillary electrophoresis, with particular interest paid to using this technique in modem pharmaceutical analytical laboratories.128 130 The most frequently used chiral selectors in CE are cyclodextrins, crown ethers, chiral surfactants, bile acids, and protein-filled... 

Y. Ito, T. Takeuchi, D. Ishii, M. Goto and T. Mizuno, Direct coupling of micro high performance liquid chromatography with fast atom bombardment mass spectrometry. II Application to gradient elution of bile acids, J. Chromatogr., 385 (1986) 201-209. 

Assay of bile acids was an essential tool for the early investigation of the enterohepatic circulation, and proved a focus of attention with the belief that serum bile-acid concentrations would provide a sensitive diagnostic test for liver disease. There are three fundamental assay types, based on enzymatic oxidation of a hydroxyl with linked NAD reduction, chromatographic separations and quantitation, encompassing both gas-liquid and high-performance liquid chromatography, and radioimmunoassay assays. 

Wang GF, Stacey NH, Earl J (1990) Determination of individual bile acids in serum by high performance liquid chromatography. Biomed Chromatogr 4 136-140... 

Lee BL, New AL, Ong CN (1997) Comparative analysis of conjugated bile acids in human serum using high-performance liquid chromatography and capillary electrophoresis. J Chro-matogr Biomed Sci Appl 704 35-42... 

Kawasaki T, Maeda M, Tsuji A (1983) Immobilized -hydroxysteroid dehydrogenase and dansyl hydrazine as a pre-labeling reagent for high-performance liquid chromatography with fluorescence detection of bile acids. J Chromatogr 272 261-268... 

Matthees, D.P. and Purdy, W.C., Naphthyldiazomethane as a derivatizing agent for the high-performance liquid chromatography detection of bile acids, Anal. Chim. Acta, 109, 161, 1979. 

Batta, A. K., Shefer, S., Batta, M., and Salen, G. (1985). Effect of chenodeoxycholic acid on biliary and urinary bile acids and bile alcohols in cerebrotendinous xanthomatosis monitoring by high performance liquid chromatography. J. Lipid Res. 26 690-698. 

Analytical techniques used to quantify either total or individual bile acids in biological fluids include gas-hquid chromatography (GLC), high-performance liquid chromatography (HPLC), enzymatic assay, radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and tandem mass spectrometry (MS/MS). 

G13. Goto, J., Saito, M., Chikai, T., Goto, N., and Nambara, T., Studies on steroids. CLXXXVIl. Determination of serum bile acids by high-performance liquid chromatography with fluorescence labeling. J. Chromatogr. 276, 289-300 (1983). 

F., Iwakawa, M., andFushimi, M., A highly sensitive determination of individual serum bile acids using high-performance liquid chromatography with immobilised enzyme. /. Chromalogr. 278, 25-34 (1983). 

R2. Reid, A. D., and Baker, P. R., High-performance liquid chromatography of bile acids with a reversed-phase radial compression column. /. Chromatogr. 247, 149-156 (1982). 

W9. Wildgrube, H. J., Fussel, U., Lauer, H., and Stockhausen. H.. Measurement of conjugated bile acids by ion-pair high-performance liquid chromatography. J. Chromatogr. 282, 603-608 (1983). 

Jensen, B.P. et al., Investigation of the metabolic fate of 2-, 3- and 4-bromobenzoic acids in bile-duct-cannulated rats by inductively coupled plasma mass spectrometry and high-performance liquid chromatography/inductively coupled plasma mass spectrometry/ electrospray mass spectrometry, Rapid Commun. Mass Spectrom., 19, 519, 2005. 

Separation of bile acid-CoA derivatives from unreacted coenzyme A can be effected by high-performance liquid chromatography. With a radial compression C,g column containing 5-/tm particles, coenzyme A was eluted with or immediately following the solvent front with a solvent system of 2-propanol/50 mM phosphate buffer (pH 7.0) (160/340) and a UV-detector set at 254 nm. Capacity factors for the above CoA derivatives are 1.50, 3.50, 4.18 and 9.50. CoA derivatives of comparable 5a-bile acids, purified and separated in a similar manner were eluted just prior to the comparable 5)8-derivative [36]. CoA derivatives of 5jS- and 5a-cholate and de-oxycholate were separable with this solvent system. 

Stubbs, R.J. Schwartz, M. Bayne, W.F. Determination of mevinolin and mevinolinic acid in plasma and bile by reversed-phase high-performance liquid chromatography. J.Chromatogr., 1986,383, 438-443... 

Gan-Schreier, H. Okun, J.G. Kohlmueller, D. Langhans, C.D. Peters, V. Ten Brink, H.J. Verhoeven, N.M. Jakobs, C. Voelkl, A. Hoffmann, G.F. Measurement of bile acid CoA esters by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). J. Mass Spectrom. 2005, 40 (7), 882-889. 

Shaw, R., Elliott, W., and Barisas, B. 1991, Estimation of critical micelle concentration of bile acids by reversed-phase high performance liquid chromatography. Mikrochim, Acta 3, 137. 

Separation of bile acid A-acetylglucosaminides by high-performance liquid chromatography with precolumn fluorescence labeling. Anal. Sci. 1992, 8, 659-662. 

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