BC coating

Figure 2.19 Schematic representation of the different hierarchical nanocomposites (from left to right, conventional plant fibers-reinforced nanocomposites, BC coated fiber-reinforced nanocomposites and BC coated fibers-reinforced hierarchical nanocomposites). Reproduced with permission from [170].

The introduction of BC onto biofibers provides new means of controlling the interaction between natural fibers and polymer matrices. Coating of biofibers with BC does not only fadHtate good distribution of BC within the matrix but also results in an improved interfadal adhesion between the fibers and the matrix. This enhances the interaction between the biofibers and the polymer matrix through mechanical interlocking. BC-coated natural fibers introduced nanocellulose at the interface between the fibers and the matrix, leading to increased stiffness of the matrix around the natural fibers [97, 98). 

BCand BC-Coated, Biofiber-Reinforced, Thermoplastic Composites I 275... 

Transfer 100 pg of streptavidin-coated microspheres (MB) into 0.5-mL Eppendorf tube. Separate, decant and wash the MB once with 100 pL of TTL buffer and then separate, decant and resuspend in 20 pL of TTL buffer. Add the desired amount of BC-A (capture DNA). Incubate the resulting solution for 15 min at temperature of 25°C and 400 rpm in a TS-100 ThermoShaker, so as to ensure immobilization. 

In a similar way, a well-adhered surface modification of BC fibers can be achieved with Ti(>2 nanoparticles (with a diameter of about 10 nm) by the hydrolysis of titanium tetraisopropanolate adsorbed onto the fibers. It was observed that the titania-coated surface appears to be dense and have low porosity and to consist of near-spherical grains. By washing with sodium carbonate solution, the TiC>2 films were not removed during neutralization. It seems that the particles have formed strong interactions with BC. The coated membranes showed substantial bactericidal properties under UV radiation and white light (containing a small fraction of UV) conditions, too. This effect is caused by the photocatalytic destruction of the bacterial cells. 

Our second on-line radioactivity detector consisted of a plastic scintillator material (BC-400, Bicron Corp., Newbury, OH) that was machined from 1-inch-diameter rod stock into a 5/8-inch-diameter (front face) solid parabola (see Figure 2). A special rotating holder was constructed for the plastic scintillator and the curved outer surfaces were coated by vacuum deposition with a thin film of aluminum in order to reflect the emitted light toward the front face of the scintillator. A detection length of 2 mm was defined within the parabola by aluminum mounting rods (0.250 inch outer diameter) that were press-fit (coaxial to the separation capillary) in the sides of the scintillator, as illustrated in Figure 2. 

In analogy with constrained peptide libraries, several reports have described the use of small proteins, protein domains, or antibodies as scaffolds for the display of random polypeptide sequences to obtain novel binding proteins or antibodies. Koide et al. (81) used the tenth FN3 sequence, a 94-amino-acid fibronectin domain (82, 83) known to be involved in molecular recognition, as a scaffold to build a phagemid 3+3 library L7 (Fig. 10.16) where less than a copy of modified FN3 was present on each phage capsid. The 10 -member library was screened using plates coated with ubiquitin, a small protein for which native FN3 does not have any affinity. The library was made by randomizing five amino acids in positions 26-30 (BC) and five amino acids in... 

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