Bacteria media preparation

Both methods, however, have disadvantages. Biochemical transformations can have limited application, and there is always the problem of finding the proper bacteria, animal preparation, or enzyme and culture medium to effect a new synthesis. In addition, product isolation—such as in the production of an optically active < -deuteroalcohol, where a small amount of product must be isolated from a large quantity of spent fermentation liquor—can present formidable separation problems. Product isolation from enzyme systems, especially immobilized enzymes, could be much simpler, however. 

The antibacterial activity of the compounds is determined by the disc diffusion method [42]. The bacteria are cultured in nutrient agar medium and used as inoculum for the study. Bacterial cells are swabbed onto nutrient agar medium (prepared from NaCl (5.0 g), peptone (5g), beef extract powder (3g), yeast extract powder (3g), and agar (20 g) in 1000 ml distilled H2O) pH 7.5 0.2 in Petri dishes. The test solutions are prepared in distilled water to a final concentration of 1 %, 2%, and 4% and then appHed to filter paper discs (Whatman No. 4, 5 mm diameter). These discs are placed on the already seeded plates and incubated at 35 2 °C for 24 h. The zones of inhibition around the discs are measured after 24 h. Co-trimoxazole is used as a standard positive control (Table 6.9). 

In the approach followed in this invention [29], a biocatalytic agent converts the sulfur heterocycles into different molecules that do not exhibit the hydrophobic interactions. This is achieved by selectively cleaving carbon-sulfur bonds. The selectivity of the biocatalytic agent employed is limited to the carbon-sulfur bonds and no attack to the carbon-carbon skeleton was reported. Thus, it is expected that the proposed biocatalytic reduction of viscosity would not diminish the fuel value of the treated petroleum liquids. The biocatalyst employed consisted of the strain ATCC No. 53968 (see Section 20 and references therein), in an aqueous culture conventionally prepared by fermentation under aerobic conditions. The fermenting bioreactor is fed with a suitable nutrient medium, which comprises a conventional carbon source (dextrose and glycerol are recommended carbon sources. To confer maximal biocatalytic activity for the desired cleavage of organic C—S bonds, the bacteria was kept in a state of sulfur deprivation. 

Syntheses of uptake hydrogenases of cyanobacteria, as well as uptake hydrogenases of other bacteria, have been shown to be dependent on the availability of Ni in the growth medium. The enzymes are insensitive to O2 in whole-cell preparations, but become sensitive after cell extraction (Houchins and Burris 1981b). 

The medication is a sterile water extract from autotrophic bacteria Ferrooxidans spp. culture medium. It is a reddish liquid with acidic reaction (pH 3.0) and astringent action. The preparation does not cause irritation of tissues and is intended for external use. However, at present the possibility of its oral administration is being studied. 

The plasma membrane of bacterial cells, other than the wall-less mycoplasmas and some archaebacteria, is surrounded by a multilayered wall which may be separated from the membrane by a thin periplasm (or periplasmic space). This can be seen most clearly in suitably prepared thin sections of cells of E. coli or other gram-negative bacteria as a relatively empty space of 11- to 25-nm thickness (Fig. 8-28).579 581 The volume of this space (which may be filled with gelled material) depends upon the osmotic pressure of the medium. In E. coli it contains 20-40% of the total... 

Inoculate either 10 pL of pooled, scraped bacteria (HB2151 or TGI depending on the library), or single colonies into 2X TY-AMP-0 1% GLU (1 mL to 1 L medium, depending on the size of preparation needed)... 

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