Uptake hydrogenase

N. A. Worin, T. Lissolo, A. Colbeau, P. M. Vignais (1996) Increased hydrogen photoproduction by Rhodobacter capsulatus strains deficient in uptake hydrogenase, J. Mar. Biotechnol., 4 28-33... 

R. Axelsson, F. Oxelfelt, P. Lindblad (1999) Transcriptional regulation of Nosloc uptake hydrogenase. FEMSMicrobiology Letters, 170 77-81... 

T. Happe, K. Schiitz, H. Bohm (2000) Transcriptional and mutational analysis of the uptake hydrogenase of the filamentous cyanobacterium Anabaena variabilis ATCC 29413.. /. Bacteriology, 182 1624-1631... 

Cyanobacteria may possess several enzymes directly involved in H2 metabolism nitrogenase(s) catalyzing the production of H2 concomitantly with the reduction of nitrogen to ammonia, an uptake hydrogenase, catalyzing the consumption of H2 produced by the nitrogenase, and a bidirectional hydrogenase, which has the capacity to both take up and produce H2 [3, 5],... 

Group 3. The NiFe-(thylakoid) uptake hydrogenases of Cyanobacteria... 

Syntheses of uptake hydrogenases of cyanobacteria, as well as uptake hydrogenases of other bacteria, have been shown to be dependent on the availability of Ni in the growth medium. The enzymes are insensitive to O2 in whole-cell preparations, but become sensitive after cell extraction (Houchins and Burris 1981b). 

In Anabaena sp. strain PCC7120, the uptake hydrogenase is less sensitive to carbon monoxide inhibition than the bidirectional enzyme. Both enzymes have low for H2 but only the uptake hydrogenase activity was elicited by addition of H2 to the gas phase. 

Axelsson, R., Oxelfelt, E. and Lindblad, P. (1999) Transcriptional regulation of Nostoc uptake hydrogenase. FEMS Microbiol. Lett., 170, 77-81. 

Elsen, S., Dischert, W., Colbeau, A., Bauer C. E. (2000) Expression of uptake hydrogenase and molybdenum nitrogenase in Rhodobacter capsulatus is coregulated by the RegB-RegA two-component regulatory system./. Bacteriol., 182, 2831-7. 

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