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Bacmid

A. californica NPV (AcMNPV Bacmid bGFP) 5. frugiperda (Sf-AE-21) Not given Not given 57 Piljman et al., 2001... [Pg.469]

Polystyrene test tubes used for bacmid transfection. CellFECTIN-DNA complex stick to the walls of polypropylene tubes, so polystyrene should be used. [Pg.25]

Prepare the following solutions in 12 x 75-mm sterile polystyrene tubes Tube 1 Add 5 pL of Bacmid DNA from Subheading 3.2. to 100 pL of serum-free TNM-FH Tube 2 Add 5 pL of CellFECTIN reagent to 100 pL of serum-free TNM-FH. [Pg.29]

We usually transfect only two recombinant bacmid DNA obtained from independent clones. We always obtain desired recombinant virus from both. [Pg.31]

Alternative methods have been developed to obtain virus stocks without plaque purification for expression of recombinant proteins in infected insect cells. The Bac-to-Bac technology (Invitrogen, Thermo Fisher Scientific) avoids homologous recombination in insect cells by using site-specific transposition in E. coli. With this technology, recombinant bacmid-DNA is generated that is used to transfect insect cells. [Pg.97]

Baculovirus infected Spodopterafrugiperda (Sf9) insect cells represent an appropriate expression system for production of membrane proteins and complexes in many applications including biophysical studies and structure determination. The Bac-to-Bac Baculovirus Expression System (Invitrogen) is used to generate bacmids and baculovirus. [Pg.400]

Recombinant bacmids separately carrying the genes for the receptor and the chemokine are generated by transforming the pFastBac constructs into DHlOBac competent Escherichia coli cells (Invitrogen) according to... [Pg.400]

The methods described below outline (1) the construction of a representative transfer vector in E. coli, (2) co-transfection of insect cells with this transfer vector and bacmid, (3) propagation of recombinant baculovirus, (4) induction of protein expression, (5) the extraction of the protein from insect cells, (6) the printing of a protein microarray, (7) the assay of a protein microarray for protein kinase activity, and (8) the assay of a protein microarray for cytochrome P450 turnover activity. [Pg.137]

See other pages where Bacmid is mentioned: [Pg.551]    [Pg.49]    [Pg.24]    [Pg.25]    [Pg.28]    [Pg.28]    [Pg.29]    [Pg.29]    [Pg.9]    [Pg.239]    [Pg.240]    [Pg.240]    [Pg.401]    [Pg.401]    [Pg.136]    [Pg.139]    [Pg.188]    [Pg.67]    [Pg.573]   
See also in sourсe #XX -- [ Pg.136 , Pg.137 , Pg.139 , Pg.188 ]



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Recombinant bacmids

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