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Recombinant bacmids

We usually transfect only two recombinant bacmid DNA obtained from independent clones. We always obtain desired recombinant virus from both. [Pg.31]

Alternative methods have been developed to obtain virus stocks without plaque purification for expression of recombinant proteins in infected insect cells. The Bac-to-Bac technology (Invitrogen, Thermo Fisher Scientific) avoids homologous recombination in insect cells by using site-specific transposition in E. coli. With this technology, recombinant bacmid-DNA is generated that is used to transfect insect cells. [Pg.97]

Recombinant bacmids separately carrying the genes for the receptor and the chemokine are generated by transforming the pFastBac constructs into DHlOBac competent Escherichia coli cells (Invitrogen) according to... [Pg.400]

The methods described below outline (1) the construction of a representative transfer vector in E. coli, (2) co-transfection of insect cells with this transfer vector and bacmid, (3) propagation of recombinant baculovirus, (4) induction of protein expression, (5) the extraction of the protein from insect cells, (6) the printing of a protein microarray, (7) the assay of a protein microarray for protein kinase activity, and (8) the assay of a protein microarray for cytochrome P450 turnover activity. [Pg.137]

See other pages where Recombinant bacmids is mentioned: [Pg.24]    [Pg.28]    [Pg.28]    [Pg.29]    [Pg.9]    [Pg.401]    [Pg.24]    [Pg.28]    [Pg.28]    [Pg.29]    [Pg.9]    [Pg.401]    [Pg.551]    [Pg.49]    [Pg.188]    [Pg.573]   
See also in sourсe #XX -- [ Pg.400 , Pg.401 ]



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