B„ cell bioassay

KLEIN K O, BARON J, COLLI M J, MCDONNELL D P and CUTLER G B, Estrogen levels in childhood determined by an ultrasensitive recombinant cell bioassay , J Clin Invest 1994 94 2475-80. 

Dinan L. reported that three resveratrol trimers, suffruticosols A (100), B (101), C (102), and one monomer cis resveratrol from Paeonia suffruticosa are active as ecdysteroids (antagonists (EDso) 10-50 pM vs. 5xlO 8pM of 20-hydroxyecdyson), but inactive as agonists in the Drosophila melanogaster BII cell bioassay for ecdysteroids agonists/ antagonists [75]. 

Nagy S R, Sanborn J R, Hammock B D (2002). Development of a green fluorescent protein-based cell bioassay for the rapid and inexpensive detection and characterization of Ah receptor agonists. Toxicol. Sci. 65 200-210. 

Paris F, Servant N, Tdrouanne B, et al. (2002). A new recombinant cell bioassay for ultrasensitive determination of serum estrogenic bioactivity in children. J. Clin. Endocr. Metab. 87 791-797. 

Okomura, M., Tsuzuk, H., and Tomita, B-I. A rapid detection method for paralytic shellfish poisoning toxins by cell bioassay. Toxicon, 46, 93, 2005. 

Continuous culture systems have been widely used to culture microorganisms for industrial and research purposes (Kubitschek 1970 Tempest 1970 Veldkamp 1976 Rhee 1980). In recent years, these culture techniques have found their way into the bioassay methods of ecotoxicology and allelopathy (Rhee 1980). The early development of a continuous culture system can be traced back to the work of Novik and Szilard (1950 a,b) who developed the first chemostat. In a continuous culture system, nutrients are supplied to the cell culture at a constant rate and to maintain a constant volume, an equal volume of cell culture is removed. This allows the cell population to reach a steady state, where the growth rate and the total number of cells/ml of culture remains constant. Two kind of continuous culture systems can be distinguished turbidostat and chemostat. ... 

Mirocha CJ, Schauerhamer B, Pathre SV (1974) Isolation, detection, and quantification of zearalenone in maize and barley. J Assoc Anal Chem 57 1104-1110 Muller R, Baier M, Kaiser WM (1991) Differential stimulation of PEP-carboxylation in guard cells and mesophyll cells by ammonium or fusicoccin. J Exp Bot 42 215-220 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15 473-497... 

Durant and co-workers (1998) analyzed an organic extract of the NIST reference complex mixture SRM 1649 (see Box 10.3) using the same human cell line (hlAlv2) and bioassay-directed fractionation. In the nonpolar fraction, cy c 1 o pe n t a[ cz/ ] py re n e, benzo[a]-pyrene, and benzo[ b ]fl uo ran the ne were responsible for 7, 4, and 2%, respectively, of the total extract mutagenicity. Only one potent O-PAC mutagen was identified in the semipolar fraction, the ketone, 6H-benzo[cd ]pyren-6-one ( 0.5%) (see discussion in Section D.3). 

Hannigan, M. P., G. R. Cass, B. W. Penman, C. L. Crespi, A. L. Lafleur, W. F. Busby, Jr., W. G. Thilly, and B. R. T. Simoneit, Bioassay-Directed Chemical Analysis of Los Angeles Airborne Particulate Matter Using a Human Cell Mutagenicity Assay, Environ. Sci. Technol., 32, 3502-3514 (1998). 

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