Auxin binding

Henderson J, Bauly JM, Ashford DA, Oliver SC, Hawes CR, Lazarus CM, Venis MA, Napier RM. Retention of maize auxin-binding protein in the endoplasmic reticulum quantifying escape and the role of auxin. Planta 1997 202 313-323. 

Napier, R. M., and M. A. Venis, From auxin-binding protein to plant hormone receptor Trends Biochem. Sci. 16 72— 75, 1991. 

Napier, R.M. Venis, M.A. (1993). Tansley Review No. 79. Auxin action and auxin binding proteins. New Phytol. 129, 167-201. 

Palme, K., Feldwisch, J., Peters, W.S., Schell, J., Zettl, R., Campos, N., Felle, H. (1992). Auxin binding proteins are located in the ER and in the plasma membrane Identification by photoaffinity-labelling and characterization. Progress in Plant Growth Regulation (Karssen, C.M., Van Loon, L.C., Vreugdenhil, D., Eds.), pp. 73-81. Kluwer Academic, The Netherlands. 

Venis, M. (1977). Solubilization and partial purification of auxin-binding sites of com membranes. Nature (London) 266, 268-269. 

Ray, P. M. Specificity of auxin-binding sites on maize cole-optile membranes as possible receptor sites for auxin action. Plant Physiol., 1977, 60, 585-591. 

Amid an ever clearer picture of auxin action, the auxin receptor remained a conspicuous omission from signaling models. Early biochemical efforts to isolate auxin receptors identified AUXIN-BINDING PROTEIN1 (ABP1).184,185 However, the majority of ABP1 localizes to the endoplasmic reticulum where the pH is too high for auxin binding. Furthermore, embryo-lethal null mutants and the lack of weak mutant alleles complicated detailed analysis. 

Auxin Binding Protein Receptor 1LR5 His 1 HiSj 3 Glu 42 His H20... 

Lejohn, H. B., A rapid and sensitive auxin binding system for detecting N6-substituted adenines, and some urea and thiourea derivatives, that show cy-tokinin activity in cell division tests, Can. J. Biochem., 53[7], 768,1975. 

Hertel, R., Thomson, K.-S., and Russo, V.E.A. 1972. In vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta, 107, 325-340. 

Auxins are synthesized in apical buds (at the tip) of growing shoots. They stimulate growth of the main shoot and inhibit lateral shoot development. A class of auxin-binding membrane proteins may represent auxin receptors. Auxin action involves pumping protons out of the cell, possibly in conjunction with a membrane ATPase. 

ABP Auxin binding protein MeJA Methyl jasmonate... 

There are three major classes of synthetic auxins the aryl acetic acids, which include indoleacetic acid (lAA) itself (Fig. 1 structure 2-1) and 1-naphthyl acetic acid (2-4), phenoxy acetic acids, represented by 2,4-dichlorophenoxy acetic acid (2,4-D) (2-7) and the benzoic acids, e.g. 2,3,6-trichloro benzoic acid (2-9). It remains the case however, that there is no stmcture-activity proposal which satisfactorily covers all molecules which are known to have auxin activity. As is well known, auxins have multiple effects, including promotion of cell elongation, cell division and gene expression. There may well be different receptors for each effect, and differences between receptors. An auxin binding protein (ABP), has now been characterised, and is possibly a receptor controlling cell elongation [3]. 

ABP auxin binding protein GAP GTPase activating protein... 

ABPl. Maize ABPl was first characterized by in vitro auxin-binding studies in the 1970s and called auxin-binding site 1 [22-24], The protein was purified by Lobler and Klambt... 

Several auxin-binding proteins (ABPs, 20,000-22,000) have been identified. These small proteins are characterized by the presence of an auxin-binding motif, a glycosylation site, and the carboxy-terminal sequence -Lys-Asp-Glu-Leu-COOH. At least 5 ABPs are present in Zea mays. In the Baculovirus expression systetrt, Zea ABPl (ZrnABPl) is targeted to the endoplasmic reticulum of the insect cells used for... 

This batch-cultured cell line apparently lacks the membrane-bound auxinbinding protein [6]. It thus provides us with a good auxin-target system to study the role of soluble auxin-binding sites in the auxin perception-transduction mechanism. Auxin-starved early-stationary phase cells respond relatively rapidly to added auxin with cell division. 

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