Electronic autoradiography

Compounds that are radioactive can be located on a preparative layer by contact film autoradiography, electronic autoradiography, and storage phosphor screen imaging [21-23]. These methods differ in terms of factors such as simplicity, speed, sensitivity, and resolution, and the method of choice depends on the available equipment, reagents, and instrumentation. All are nondestructive, and the detected compounds can be recovered without change for later studies. 

Wolfe, D. E., Potter, L. T., Richardson, K. C. and Axelrod, J. Localizing tritiated norepinephrine in sympathetic axons by electron microscopic autoradiography. Science 138 440M42, 1962. 

Baraban, J. M., and Aghajanian, G. K. (1981) Noradrenergic innervation of serotonergic neurons in the dorsal raphe Demonstration by electron-microscopic autoradiography. Brain Res., 204 1 — II. 

Evans LV, Callow ME. Autoradiography, in Electron Microscopy and Cytochemistry of Plant Cells (Hall JL, ed.), Elsevier/North-Holland, Amsterdam, The Netherlands, 1978, pp. 235-277. 

Domer P. Photometric methods in quantitative autoradiography, in Microautoradiography and Electron Probe Analysis. Their Application to Plant Physiology (Luttge U, ed.), Springer Verlag, New York, 1972, pp. 7M8. 

Fakan S, Fakan J. Autoradiography of spread molecular complexes, in Electron Microscopy in Molecular Biology. A Practical Approach (Sommerville J, Scheer U, eds.), IRL Press, Oxford, UK, 1987, pp. 201-214. 

SigreDC. Electron microscope autoradiography. PureAppl Chem 1991 63 1277-1283. 

C. These freeze-dried sections were dry mounted on microscope slides which had been precoated with either Kodak NTB-3 or NTB-10 emulsion. Other techniques which thawed the frozen section, embedded the tissue in paraffin or dipped the section in liquid emulsion were demonstrated to translocate diffusible compounds. Many other similar attempts have been and are currently being made to localize diffusible compounds by autoradiography at the electron microscope level. 

Littau VC, Allfrey VG, Frenster JH, Mirsky AE (1964) Active and inactive regions of nuclear chromatin as revealed by electron microscope autoradiography. Proc Natl Acad Sci USA 52 93-100 Longmuir KJ, Robertson RT, Haynes SM, Baratta JL, Waring AJ (2006) Effective targeting of liposomes to liver and hepatocytes in vivo by incorporation of a Plasmodium amino acid sequence. Pharm Res 23 759-769... 

Figure 5.10. Accumulation of a radiolabelled LMWP in the lysosomes of the proximal tubular cell. Electron microscope autoradiography of renal proximal tubular cells from a rat injected i.v. with [1251]-tyramine-cellobiose-labelled cytochrome-c, 4 h prior to fixation throngh the abdominal aorta. An intense lysosomal accumulation of the protein is observed in three dark electron-dense lysosomes. A few grains are seen over the apical endocytic apparatus. Part of the luminal brush border is found in the upper right hand corner. Magnification, x 25 000. Unpublished data from E. I. Christensen, Arhus, Denmark, and M. Haas, Groningen, Netherlands.

Lupulescu A. 1973. Electron microscopic autoradiography of 3H-kerosene in human epidermis 13th annual meeting of American Society for Cell Biology. Journal of Cell Biology 59(2). 

J. T. Greenamyre, D.S. Higgins, R.V. Ellers, Quantitative autoradiography of dihydror-otenone binding to complex I of the electron transport chain, J. Neurochem. 59 (1992) 746-749. 

FIGURE 25-3 Visualization of bidirectional DNA replication. Replication of a circular chromosome produces a structure resembling the Greek letter theta (0). (a) Labeling with tritium (3H) shows that both strands are replicated at the same time (new strands shown in red). The electron micrographs illustrate the replication of a circular E. coli plasmid as visualized by autoradiography, (b) Addition of 3H for a... 

Bienz, K (1977) Techniques and applications of autoradiography m the light and electron microscope Microsc Acta 79, 1-22... 

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