Electron microscopy autoradiography

The results will rely on the sampling method (individual organ or whole-body study) and on the techniques used (paraffin embedding, cryomicrotomy, light or electron microscopy, autoradiography...)... 

Evans LV, Callow ME. Autoradiography, in Electron Microscopy and Cytochemistry of Plant Cells (Hall JL, ed.), Elsevier/North-Holland, Amsterdam, The Netherlands, 1978, pp. 235-277. 

Fakan S, Fakan J. Autoradiography of spread molecular complexes, in Electron Microscopy in Molecular Biology. A Practical Approach (Sommerville J, Scheer U, eds.), IRL Press, Oxford, UK, 1987, pp. 201-214. 

Autoradiography is a technique for locating radioactive compounds within cells it can be conducted with light or electron microscopy. Living cells are first exposed to the radioactive precursor of some intracellular component. The labeled precursor is a compound with one or more hydrogen ( H) atoms replaced by the radioisotope tritium (3H) e.g., [3H]thymidine is a labeled precursor of DNA, and [3H]uridine is a labeled precursor of RNA (Chap. 7). Various tritiated amino acids are also available. The labeled precursors enter the cells and are incorporated into the appropriate macromolecules. The cells are then fixed, and the samples are embedded in a resin or wax and then sectioned into thin slices. 

Bolam JP, Clarke DJ, Smith AD, Somogyi P (1983) A type of aspiny neuron in the rat neostriatum aecumu-lates [ Hjgamma-aminobutyric acid combination of Golgi-staining, autoradiography, and electron microscopy. J. Comp. Neurol, 213, 121-134. 

When DNA replication was first observed experimentally (using electron microscopy and autoradiography), investigators were confronted with a paradox. The bidirectional synthesis of DNA as it appeared in their research seemed to indicate that continuous synthesis occurs in the 5 —> 3 direction on one strand and in the 3 —> 5 direction on the other strand. (Recall that DNA double helix has an antiparallel configuration.) However, all the enzymes that catalyze DNA synthesis do so in the 5 —> 3 direction only. It was later determined that only one strand, referred to as the leading strand, is continuously synthesized in the 5 —> 3 direction. The other strand, referred to as the lagging strand, is also synthesized in the 5 — 3 direction but in small pieces (Figure 18.6). (Reiji... 

M. A. Williams, Autoradiography and Immunocytochemistry , in Practical Methods in Electron Microscopy, Vol 6 (ed. A. M. Glauert), North-Holland, Amsterdam, 1977. 

V. M. PickelandA. Beaudet, "Combined Use of Autoradiography and Immunocytochemistry to Show Synaptic Interactions Between Chemically Defined Neurons, in Immunolabeling for Electron Microscopy (eds. J. M. Polak and I. M. Varndell), Elseviser, Amsterdam, 1984, pp. 259-266. 

The extent of lumen penetration into earlywood, latewood and wood rays is preferably determined by examination of the cross section of the bondline, and many techniques have been successfully used for this purpose light microscopy [29, 30], transmitted and reflected microscopy [31-33], (epi-)fluorescence microscopy [2, 10, 26, 31, 34-40], fluorescence confocal laser scanning microscopy (CLSM) [34, 41 5], scanning electron microscopy (SEM) [32, 46 8], transmission electron microscopy (TEM) [49-52], SEM in combination with an energy-dispersive analyzer for X-rays (SEM/EDAX) [43, 53], X-ray microscopy [54], autoradiography [11] or combination of different microscopy techniques [55]. Kamke... 

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