Automation kinetics

Becer CR, Groth AM, Paulus RM et al. (2008) Protocol for automated kinetic investi-gation/optimization of the RAFT polymerization of various monomers. QSAR Comb Sci 27 977-983... 

Green C, McKee S, Saunders K A fully automated Kinetic solubility screen in 384-well Plate format usinig nephelometry. BMG, Application Note 117... 

These systems are designed for the endpoint determination of substrate concentrations, and do not provide a straightforward means for making kinetic measurements. Stopped-flow enzyme reactor systems have been designed for automated kinetic assays. A diagram of a stopped-flow reactor that uses a postcolumn chemical indicator reaction is shown in Figure 4.12.31 In this system, the flow rate of the... 

Knapp ML, Mayne PD, Development of an automated kinetic Jaffe method designed to minimize bilirubin interference in plasma creatinine assays. 

Schwedtfeger. The Automated Kinetic Assay of Solanine. ISHS Acta Horticulturae 163. 

Although the MM equation is a powerful kinetic form to which the vast majority of enzyme kinetics has been fitted, one should not forget the assumptions and limitations of the model. As a basic example, feedback inhibition, whereby the product of the reaction inhibits the enzyme-substrate cooperativity, multiple-substrate reactions, allosteric modifications, and other deviations from the reaction scheme in equation (1) are treated only adequately by the MM formalism under certain experimental conditions. In other words, enzyme kinetics are often bent to conform to the MM formalism for the sake of obtaining a set of parameters easily recognizable by most biochemists. The expUcit mathematical and experimental treatment of reaction mechanisms more complex than that shown in equation (1) is highly involved, although a mathematical automated kinetic equation derivation framework for an arbitrary mechanism has been described in the past (e.g., ref. 6). 

An automated kinetic assay for the determination of D-glucose in blood has employed D-glucose dehydrogenase [EC 1.1.1.47] in the presence of muta-rotase and nicotinamide adenine dinucleotide as hydrogen acceptor. The technique is highly specific and can be applied to blood and other body fluids. 

Perez-Bendito D, Silva M, and Gomez-Hens A (1989) Automated kinetic-based determinations for routine analyses recent developments. Trends in Analytical Chemistry 8 302-308. 

The features of environmental samples make them especially suitable for automated kinetic analyses. The determination of metal ions in environmental samples is of great ecological significance as these species cannot be degraded biologically or chemically. Kinetic methods are useful tools for determining heavy metals in environmental water and air, where toxic species occur at low concentration levels. Table 6 collates some relevant applications, among which those concerned with water analyses are prominent. 

The software was rewritten in Java to make it possible to be used with the multiplicity of operating systems now available in the lab, and data is collected using a simple USB connection. Furthermore, the ability to automate kinetic measurements has also been added. 

Hungerford, J. M., T. A. Hollingworth, and M. M. Wekell. 2001. Automated kinetics-enhanced flow injection method for histamine in regulatory laboratories Rapid screening and suitability requirements. Anal. Chim. Acta 438 123-129. 

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