Attenuated total reflection dichroism

Abbreviations CCK, cholecystokinin RNase. ribonuclease G-protein, guanine nucleotide binding protein GPCR, G-protein-coupled receptor. SDS sodium dodecylsulfate, CTAH. hexadecyltrimethyl ammonium hydroxide DMPC. di-myristoylphosphatidylcholine DPPC, di-palmitoylphosphatldylcholine CMC, critical micellar concentration SUV, small unilamellar vesicles CD, circular dichroism NMR, nuclear magnetic resonance hs-DC, high sensitivity differential scanning calorimetry IR-ATR, infrared attenuated total reflection spectroscopy NOE, nuclear Overhauser effect MD, molecular dynamics DMSO, dimethylsulfoxide TFE, trifluoroethanol for abbreviations of peptides see tables land 2, and fig. 11. [Pg.820]

Applied Spectroscopy 51, No.8, Aug.1997, p.1083-91 IMPROVEMENTS IN USE OF ATTENUATED TOTAL REFLECTION FOURIER TRANSFORM INFRARED DICHROISM FOR MEASURING SURFACE ORIENTATION IN POLYMERS Everall N J Bibby A... [Pg.84]

The stmctural and conformational analysis of proteins adsorbed to solid surfaces is difficult because most common analytical methods are not compatible with the presence of the interacting solids. With recent developments in instrumentation and techniques, our understanding of protein adsorption behavior has improved considerably [4, 14]. The most commonly used techniques include attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), radiolabeling techniques, immunofluorescence enzyme-linked immunosorbent assay (ELISA), ellipsometry, circular dichroism (CD) spectroscopy, surface plasmon resonance (SPR), and amide HX with nuclear magnetic resonance (NMR). Atomic force microscopy (AFM) and scanning... [Pg.266]

Attenuated total reflection spectroscopy Atomic X-ray absorption fine structure spectroscopy Circular dichroism... [Pg.284]

Attenuated total reflection (ATR) FTIR is one of the most useful tools for characterising the chemical composition and physical characteristics of polymer surfaces [53]. One useful application is the measurement of molecular orientation using polarised infrared ATR spectroscopy [54,55]. The polarised infrared ATR spectra normally include three-dimensional (e.g., machine, transverse, and thickness direction) orientational information in contrast to the polarised transmission infrared linear dichroism. In addition, band absorbance of less than 0.7 au is easily achieved, even with the strong absorption bands, because the penetration depth of ATR from sample surfaces can be adjusted to a few micrometers by changing the internal reflection element and/or the angle of incidence. If successful combination of the dynamic infrared spectroscopy and the ATR methods can be achieved, more useful dynamic orientational information can be obtained. [Pg.292]

Everall, N.J. and Bibby, A. (1997) Improvements in the use of attenuated total reflection Fourier transform infrared dichroism for measuring surface orientation in polymers. Appl Spectrosc., 51, 1083—1091. [Pg.440]

Another useful dichroic technique is attenuated total reflection (atr) dichroism, which provides the surface dichroism of oriented products (106-108). [Pg.899]

Attempts to elucidate the recognition mechanism acting in polysaccharide-derived CSPs have been made. However, despite the powerful computational modeling methods, and spectroscopic techniques available at present (NMR, X-ray diffraction (XRD), vibrational circular dichroism (VCD), attenuated total reflectance infrared spectroscopy (ATR-IR), among those more frequently used), it remains unknown at the level required for prediction (Figme 54.3). ... [Pg.1605]