Arthrobacter aurescens

The initial steps in the degradation of 2 -methyl-4-methoxystyrene (tran -anethole) by a strain of Arthrobacter aurescens are comparable to those already noted, except that the epoxide is hydrolyzed to a diol to produce 4-methoxybenzoate (Shimoni et al. 2002). 

Strong LC, C Rosendahl, G Johnson, MJ Sadowsky, LP Wackett (2002) Arthrobacter aurescens TCI metabolizes diverse s-triazine ring compounds. Appl Environ Microbiol 68 5973-5980. 

Sajjaphan, K., N. Shapir, L.P. Wackett, M. Palmer, B. Blackmon, J. Tomkins, and MJ. Sadowsky (2004). Arthrobacter aurescens TCI atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli. Appl. Environ. Microbiol., 70(7) 4402 4407. 

Shapir, N., C. Rosendahl, G. Johnson, M. Andreina, M.J. Sadowsky, and L.P. Wackett (2005b). Subshate specificity and colorimetric assay for recombinant trzN derived from Arthrobacter aurescens TCI. Appl. Environ. Microbiol., 71(5) 2214—2220. 

Source Arthrobacter crystallo- Arthrobacter aurescens Pseudomonas fluorescens Pseudomonas striata Pseudomonas sp. A)... 

Additionally, an Escherichia coli whole cell biocatalyst has been constructed containing the genes of hydantoinase, hydantoin racemase and i-N-carbamoylase from Arthrobacter aurescens in optimal proportions, so that during the reaction no l-N-carbamoyl amino acid occurs as an intermediate product any longer11431. 

While an isolated soil bacterium, Arthrobacter aurescens, had been found to secrete a chondroitin lyase AC into the culture medium, it was recognized that the condroitinase preparation obtained from the broth of the strain cultured in a jar fermenter contained at least three electrophoretically separable components... 

Fh, Flavobacterium heparinum Aa, Arthrobacter aurescens Pv, Proteus vulgaris Bs, Bacteroides stercoris Pa, Propionibacterium acnes... 

The chondroitin sulphate lyase AC (mol. wt. 7.5—8.0 x 10" ) from Arthrobacter aurescens is a glycoprotein containing residues of D-mannose, D-glucose, 2-amino-2-deoxy-D-glucose, and D-glucuronic acid (molar proportions 3 5 4 2). The amounts of a-helix and -structure present in the protein chain were estimated to be 16 and 25 %, respectively, by o.r.d. and c.d. spectroscopy, etc. 

Arthrobacter aurescens TCI strain that was isolated by direct plating of contaminated soil on atrazine fortified agar plates. These results may suggest that selective enrichment in liquid media preferentially enriches for Gramnegative atrazine-degrading bacteria with catabolic pathways consisting of atzABC,... 

Arthrobacter aurescens secretes an enzyme that hydrolyses chondroitin sulphate. The enzyme crystallized after purification and exhibited the following properties pH optimum 6.0, temperature optimum 50 C, and pH stability 4.9—7.4 it was stable below 45 °C and hydrolysed chondroitin 4- and 6-sulphates, chondroitin, and hyaluronic acid (ratio of initial rates 1.0 1.1 1.95 3.2). The hydrolyses of chondroitin 4- and 6-sulphates furnished 2-acetamido-2-deoxy-3-0-(4-deoxy-a-L-t/irco-hex-4-enopyranosyluronic acid)-D-galactose 4- and 6-sulphates, respectively. Since the enzyme is inactive against dermatan sulphate, it is a chondroitin sulphate lyase AC. 

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