Armour Laboratories

The protein used was crystalline bovine serum albumin (BSA) obtained from Armour Laboratories. Sodium dodecyl sulfate (SDS) was synthesized from pure dodecyl alcohol and chlorosulfonic acid, recrystallized, and washed with ethyl ether. Sulfuric acid was reagent grade purchased from the J. T. Baker Chemical Co. and used directly. Reagent grade potassium hydroxide purchased from the Mallinkrodt Co. was purified by foaming a concentrated solution, removing the foam, and using the solution directly. 

Proteins. Crystalline lysozyme, bovine serum albumin, ribonucle-ase,and pepsin were obtained from the Armour Laboratories and crystalline /3-lactoglobulin was prepared from skimmed milk. a-Lactalbu-min was obtained from W. G. Gordon of this laboratory. Crystalline ovalbumin and human serum albumin were obtained from Nutritional Biochemical Corp. and purified pigskin gelatin from the Eastman Kodak Co. 

For enzymic hydrolysis (40), 0.1 g. of protein material is dissolved or suspended in 1 ml. of 0.1% NaCl, adjusted to pH 8.5 with 0.1 N NaOH, and mixed with 1 ml. of a 0.5% solution of pancreatic proteases (unfractionated trypsin of Armour Laboratories, Chicago, is suitable for this purpose) which has been brought ko pH 8.5. One drop of toluene is added and the mixture is kept for 72 hours at 38°, 4 to 6 readjustments of the pH to 8.5 are made, and 0.5 ml. of the proteinase solution is added after 24 and after 48 hours. 

Assays for melanocyte-stimulating hormone were carried out by Mr. S. Kulovich using the in vitro frog skin assay (1). Subcutaneous assay for adrenocorticotropic activity (2), based on ascorbic acid depletion in hypophysectomized rats, was performed by Dr, J.D. Fisher of the Armour Laboratories. Acid hydrolysates (constant boiling HCl, deaerated, 22 hours, 110°) of these fractions were characterized by automatic amino acid analysis with a Spinco model 120B analyzer. The number of tryptophan residues in the intact peptide was estimated from the ultraviolet absorption curves made with a Cary model 15 spectrophotometer. Electrophoresis was carried out on Whatman paper No. 1 with pyridine-acetate buffer, pH 6.5, and 4 molar urea for 3 hours at 26 volts per cm. Peptides were detected with bromphenol blue(3). 

M.-A. Armour, Hazardous Laboratory Chemicals Disposal Guide, 2nd Edn, CRC Press, Boca Raton, 1996. ISBN 1566701082. 

See also P. Patnaik, A Comprehensive Guide to the Hazardous Properties of Chemical Substances, 2nd ed. (New York Wiley, 1999) G. Lunn and E. B. Sansone, Destruction of Hazardous Chemicals in the Laboratory (New York Wiley, 1994) and M. A. Armour, Hazardous Laboratory Chemical Disposal Guide (Boca Raton, FL CRC Press, 1991). 

N.J. Ballistics Research Laboratories at Aberdeen Proving Ground, Maryland and Armour Research Foundation, Chicago, Illinois... 

C EN 38, 63(Dec 12, 1960)(A brief description of cartridge case developed by Armour Research Foundation no composition of material is given) 13)NavaI Research Laboratory, "Plastic Combustible Cartridge Cases , NOL Repts 588(l960-1963)(These reports include description of polyethylene obturators) 14)R.F. Remaly, "Basic Research on Combustible Cartridge Cases and Associated Development Work , Armour Research Foundation... 

In a study conducted by the Armour Research Foundation (Ref 41) a-Pb azide crystals wrapped in a thin A1 foil were subjected to fast and thermal neutrons in the heavy water pile at Argonne National Laboratory. With a thermal flux rate of about 10l4n/cm2/sec the crystals were irradiated for 8, 17 and 170 hours. The crystals decompd to a brown powder which was identified as Pb carbonate by X-ray techniques and infrared absorption spectra. From a mass spectrographic analysis of the isotopes of carbon and oxygen in the decompn products, it was determined that the mechanism of carbonate formation is a reaction with the atmosphere by broken surface bands produced by the neutrons. Subsequently, Raney (Ref 60) reported... 

We wish to acknowledge the aid and cooperation of Dr. Charles H. Riesz of the Armour Research Foundation and Dr. David Miller of the Argonne National Laboratory in connection with the preparation of this volume. 

Armour, M.A., Laboratory methods for the disposal of toxic inorganic and organic chemicals, in Hazardous Waste Control in Research and Education, Korenaga, T. et al., Eds., Lewis Publishers, Boca Raton, FL, 1994, p. 102. 

Armour, M.A. and Linetsky, A., A Laboratory Disposal Method for Acrylic Acid, University of Alberta, Edmonton, 1999. 

Armour, M.A., Disposal methods for some known or suspect chemical carcinogens, in Recent Advances of Chemistry and Molecular Biology in Cancer Research, Dai, Q., Armour, M.A., and Zheng, Q., Eds., Springer-Verlag, Heidelberg, 1993, p. 315 Armour, M.A., Chemical treatment methods to minimize waste, in Pollution Prevention and Waste Minimization in Laboratories, Reinhardt, P.A. et al., Eds., Lewis Publishers, Boca Raton, FL, 1995, p. 283. 

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