Arabidopsis thaliana mutants

STASWICK, P.E., SU, W., HOWELL, S.H., Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutant, Proc. Natl. Acad. Sci. USA, 1992,89,6837-6840. 

Conklin, P.L., Saracco, S.A., Norris, S.R., and Last, R.L., 2000, Identification of ascorbic acid-deficient Arabidopsis thaliana mutants. Genetics 154 847-856. 

Goujon, T., Sibout, R., Pollet, B., Maba, B., Nussaume, L., Bechtold, N., Lu, R, Ralph, J., Mila, 1., Barriere, Y., Lapierre, G. and Jouanin, L. (2003a) A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters. Plant Mol. Biol., 51, 973-89. 

Microsomes prepared from maize (Zea mays) embryos or seedlings have proved to be an excellent biochemical system to study sterol bios)mthesis in vitro. The development of molecular genetics tools, the availability of specific Arabidopsis thaliana mutants and the possibilities to genetically transform these mutants allowed the application of a new strategy to study sterol biosynthesis pathways (Benveniste, 2004). Some important results of both approaches are summarized above (Figs 6.2 and 6.3). 

Progress in the isolation and characterization of new Arabidopsis thaliana mutants promises to unravel some elements in the hormone-cytoskeleton-plant development chain of interactions since these mutants are often characterized by altered hormonal balances (e.g. references [4—9]). The fact that morphogenesis is modified in the mutant plants does... 

Peterson RB, and Havir EA. Photosynthetic properties of aa Arabidopsis thaliana mutant possessing a defective PsbS gene. Planta 2001 214 142-152. 

Model plants Arabidopsis thaliana Range of available mutants, accessible genetics, ease of transformation Not useful for commercial production (low biomass)... 

HARTEL, H., LOKSTEIN, H., DORMANN, P TRETHEWEY, R.N., BENNING, C., Photosynthetic light utilization and xantophyll cycle activity in the galactolipid deficient dgdl mutant of Arabidopsis thaliana, Plant Physiol. Biochem., 1998,36,407-417. 

WISMAN, E., HARTMANN, U., SAGASSER, M., BAUMANN, E., PALME, K., HAHLBROCK, K., SAEDLER, H., WEISSHAAR, B., Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes, Proc. Natl. Acad. Sci. USA, 1998,95,12432-12437. 

FOCKS, N., SAGASSER, M., WEISSHAAR, B., BENNING, C, Characterization of ttl5, a novel transparent testa mutant of Arabidopsis thaliana (L.) Heynh, Planta, 1999, 208, 352-357. 

KOOKNNEEF, M., VAN DER VEEN, J.H., Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) Heynh, Theor. Appl. Genet., 1980, 58, 257-263. 

Ogas, J., Cheng, J.C., Sung, Z.R., and Somerville, C. (1997) Cellular differentiation regulated by gibberellin in the Arabidopsis thaliana pickle mutant. Science 277, 91-94. 

Yeast does not synthesise cycloartenol or triterpenes, and so approaches to clone plant OSCs by complementation in S. cerevisiae are not feasible because of the lack of appropriate mutants. However, LS-deficient yeast mutants accumulate high levels of 2,3-oxidosqualene, favouring the synthesis of novel cycfisation products generated by heterologous expression of OSCs. The absence of lano-sterol also facilitates analysis of the reaction products. Corey and co-workers isolated a cDNA encoding Arabidopsis thaliana CS by transforming a plant cDNA expression library into such a yeast mutant and screening protein preparations derived from pools of transformants for the ability to synthesise cycloartenol by TLC [39]. 

The results of a recent study have called this into question. The primary walls of a double mutant of Arabidopsis thaliana in which two xyloglucan xylosyltransferase genes were not expressed were found to contain no detectable xyloglucan (Cavalier et al., 2008). Compared to the wild type, the double mutant was slightly smaller and had abnormal root hairs, yet there was no catastrophic effect on cell-wall integrity as would be predicted from current cell-wall models. A possible explanation is that the pectic polysaccharide network between the... 

Heremans, B. Jacobs, M. A mutant of Arabidopsis thaliana (L.) Heynh. with modified control of aspartate kinase by threonine. Biochem. Genet., 35, 139-153 (1997)... 

Harpham, N.V.J. et al. (1991). The effects of ethylene on the growth and development of wild-type and mutant Arabidopsis thaliana (L) Heynh. Annals Bot. 68, 56-61. 

Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ, Masson PH, Murphy AS. 2004. Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoids mutants with altered auxin transport in Arabidopsis thaliana. Plant Cell 16 1898-1911. 

Koomneef, M., Reuling, G. Karssen, C.M. (1984). The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiologia Plantarum 61, 377-83. 

Jacobs, M., Dolferus, R. Van Den Bossche (1988). Isolation and biochemical analysis of ethyl methane sulfonate induced alcohol dehydrogenase null mutants of Arabidopsis thaliana (L.) Heynth. Biochemical Genetics 26, 105-12. 

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