Application preparative chromatography

The basis of chiral method development screening strategies is largely dictated by whether the method is to be applied to analytical or preparative applications. Preparative chromatography tends to use volatile modifiers and mobile phases for easy removal after sample collection. Sample isolation capabilities have led to the advancement of chiral SFC since CO2 is easily driven off. Chiral separations in preparative chromatography only need to be optimized to a point where pure samples can be collected. Conversely, while analytical chromatography can use a greater variety of modifiers and mobile phases, the separation needs to be optimized to a point where suitable resolution (R > 1.5) is achieved between the enantiomers of interest. 

K. Hostettmann, A. Marston and M. Hostettmann, Preparative Chromatography Techniques Applications in Natural Product Isolation, 2nd Edn, Springer-Verlag, Berlin, 1998. ISBN 3540624597. 

Nieoud R. M., Seidel-Morgenstem A. (1993) Adsorption Isotherms Experimental Determination and Applieation to Preparative Chromatography Simulated Moving Bed Basics and Applications, R. M Nieoud (ed.), Institut National Polyteehnique de Lorraine, Naney, Pranee, p. 4-34. 

These ideas and methods of preparative selective up-scale chromatography suggest that the use of new types of biosorbents and, in particular cellosorbents, and the application of theoretically based conditions for stepwise desorption of the components is an important new approach to preparative chromatography. 

Kaltenbrunner, O. and Jungbauer, A., Simple model for blending aqueous salt buffers. Application to preparative chromatography, /. Chromatogr. A, 769, 37, 1997. 

At concentrations above ( ), a phase separation would lead to the formation of particles dispersed in a liquid matrix. The composition of such particles should be given by the binodal line. Thus such particles will still contain enough solvent to undergo a phase separation. Indeed such an internal phase separation can be used to prepare porous polymeric particles with potential for application as chromatography beads [48]. 

Scale-Up and Optimization in Preparative Chromatography Principles and Biopharmaceutical Applications, edited by Anurag S. Rathore and Ajoy Velayudhan... 

Another way to obtain pure enantiomers is the separation of racemates through preparative chromatography on chiral stationary phases. In fact, the most significant developments over the last 20 years have been the application of GLC and HPLC techniques to the effective resolution of enantiomeric mixtures and to determining the enantiomeric ratio [7,8],... 

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