Classical preparative layer chromatography applicability

Classical preparative layer chromatography (PLC) is designed for the isolation of 10 to 1000 mg quantities of material. The practice of PLC and analytical TLC is similar in most respects, except that thicker layers are employed for the former. Most preparative applications have been carried out on 20 X 20 cm silica gel or alumina plates with a layer thickness of 1-5 mm and fluorescent indicator to facilitate nondestructive detection. 

Circular development of TLC plate, 27 Classical preparative layer chromatography (CPLC), 307,308-317 applicability of, 317... 

The purpose of this chapter is to present an overview of all important aspects of thin-layer chromatography (TLC). It will briefly review information and references on topics covered in the following chapters in Part I and will refer readers to these specific chapters. It will treat in more detail topics not covered further in separate chapters, such as sampling and sample preparation and the more classical procedures of TLC. A suggested source of additional basic, practical information on the practice and applications of TLC is the primer written by Fried and Sherma (1). 

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