Antisense chemistries

Based on the above screening, a modified analog of AS5-2 with advanced antisense chemistry was designed (Wang et al., 1999b). The structures of Oligo AS and its mismatched control ASM are as follows ... 

Tieman, D.M, Harriman,R.W, Ramamohan,G and Handa,A.K (1992) An antisense pectin methylesterase gene alters pectin chemistry and soluble solids in tomato fiuit. The Plant Cell. 4. 667-679. ... 

Aboul-Fadt, T. 2005. Antisense oligonucleotides the state of the art. Current Medicinal Chemistry 12(19), 2193-2214. 

Example 56 the Isis Pharmaceutical group in their extensive investigations of antisense oligonucleotides as therapeutics has described the synthesis of 3 -C-methylene nucleoside phosphonoamidites for the new backbone modification of oligonucleotides [90]. This paper gives good insight into tricoordinate phosphorus and related H-phosphonate chemistry in the service of nucleotide synthesis. 

Isis Pharmaceuticals Chemistry/Formulations, http // www.isispharm.com/Antisense-Technology/ Antisense-Drug-Discovery-Platform/Medicinal-Chemistry. htm (accessed August 2012). 

M. E. Gleave. 2001. Antitumor activity of antisense clusterin oligonucleotides is improved in vitro and in vivo by incorporation of 2 -0-(2-methoxy)ethyl chemistry. 

C. York-Defalco, H. Sasmor, B. Conklin, and R.S. Geary. 2000. Absorption of anti-sense oligonucleotides in rat intestine effect of chemistry and length. Antisense Nucleic Acid Drug Dev. 10 35-44. 

Capillary gel electrophoresis has been employed both for molecular biology applications, such as PCR product analysis and antisense gene therapy, and for protein chemistry applications such as protein separations. 

Manoharan M. Harnessing RNA interference pathways for therapeutics utilizing chemistry chemically modified siRNAs, drug delivery systems and antagomirs. In Crooke ST, ed. Antisense Drug Technology. New York Dekker, in press. 

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