Antimony III Chloride Reagent

Antimony(III) Chloride Reagent 207 The solution should always be freshly made up. 

B Vanillin-phosphoric acid reagent (VPA No. 41) —i vis C Antimony-(III)-chloride reagent (SbCljNo. 4) —> UV-365 nm... 

Detection with antimony-(III)-chloride reagent (SbCl, No. 4) blue (vis) zones (e.g. proscillaridin)... 

Antimony(III)-chloride reagent (No. 15) is used for the colour reaction. It contains 2% acetyl chloride and, for stabilisation, 2% acetic anhydride. 

Solanidine and demissidine can be separated on a silver nitrate-impregnated silica gel layer. Schbeibeb et al. [207 b] have used TLC also for preparative separation of Solanum alkaloids. Using 8 solvents on silica gel G layers, Paqijin and Lepage [158] have separated the three principal alkaloids from Solanum tuberosum, i. e., solanine, chaconine and solanidine. Antimony(III) chloride reagent was used for visualisation. P bez-Medina et al. [164] have investigated many Solanum species in the search for a raw material source of solasodine. They used TLC on silica gel 6 with n-butanol-methanol-diethylamine (85 + 5 + 10) as solvent and antimony(III) chloride (Rgt. No. 16) for visualisation. The TLC of this alkaloid class is described in other publications [2, 25, 183, 208]. 

Fluorescamines Antimony (III) chloride (10% in chloroform) Benedict reagent Lead (IV) acetate (basic, 25%) p-Toluenesulfonic acid (20% in chloroform) Perchloric acid (70%)... 

In particular, sulphuric acid, molybdophosphoric acid, antimony(III)-chloride and fluorescence indicators and others quoted in Table 58 can be used for detection. Richter [142] has obtained blue colours with Zl - and zl -3j8-hydroxysterols in amounts down to 0.03 fig, using the naphthoquinonesulphonic acid-perchloric acid reagent. Reference may be made to Boliiger s chapter (K) for the vitamin D group to section XI on bile acids for bile alcohols (neutral C27-sterols) and to Mangohd s chapter (M) for separation of lipids, including cholesterol and its esters. 

Tables 58 and 59 contain information about detection. The most popular reagents have been aromatic aldehydes-acids (Nos. 11, 48, 133, 219, 260, 262), chlorosulphonic acid-acetic acid (Rgt. No. 45), sulphuric acid (No. 241) and antimony (III)-chloride (No. 15).

Reagents Carbon tetrachloride 25% antimony(III)chloride in reagent grade chloroform (freshly prepared each day). 

Spray reagent 10% solution of antimony(III) chloride in chloro-... 

Although most steroids have their own U V-absorbance suitable for detection, localization of the spots can be based on the appearance of dark spots at 254 nm UV-light if a fluorescence layer is used most applications involve methods based on chemical reactions. Many spray reagents for the visualization of steroid spots have been described. However, special attention is needed to stabilize the color produced or the fluorescence induced on the plate. Sulfuric acid-methanol (or ethanol) is the most widely used reagent. In addition, phosphoric acid or antimony (III) chloride can be used to detect steroids on the plate or to detect steroids after spot elution. 

Evaluation reagents I ethanolic sulfuric acid, 2 tetrazolium blue, 3 antimony(III)chloride, 4 chloramine T. 5 xanihidrol, 6 phosphoric acid, 7 iodine, 8 hydrochloric acid, 9 Dragendorff reagent, 10 phosphomolybdic acid, II p-acelylaminobcnzaldehydc scmicarbazonc, 12 1,4-dihydrazinophtalazine. 

Evaluation reagents Col-1 ethanolic or methanolic sulfuric acid-l-UV254, Col-2 alkaline tetrazolium blue. Col 3 antimony/III/chloride, Col-4 perchloric acid, Col-5 p-toluene sulfonic acid, Col-6 chloramine T, Col-7 arzenomolybdate, Col-8 phosphomolibdic acid. 

Reaction of 13C-labeled chloroform with this reagent for 3 hours at O C gives 13CHFC12 as the major product. Under modified conditions, when 13CHC13 is added to the fluorinating agent [4 g of solid antimony(III) fluoride and 4 g of liquid antimony(V) chloride] and is warmed to 40 °C, the volatile product that distills off at — 90°C is 13C-labeled chlorodifluoromethane (80% conversion).73... 

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