Antibody antifluorescein

Wang R., Narang U., Prasad P.N., Bright F.V., Affinity of antifluorescein antibodies encapsulated within a transparent sol-gel glass, Anal. Chem. 1993 65 2671-2675. 

Peroxidase-conjugated mouse monoclonal antifluorescein antibody... 

Sklar, L. A., Oades, Z. G., Jesaitis, J., Painter, R. G., and Cochrane, C. G. (1981). Fluoresceinated chemotactic peptide and high-affinity antifluorescein antibody as a probe of the temporal characteristics of neutrophil stimulation. Proc. Natl. Acad. Sci. USA 78, 7540-7544. 

Hatanaka Y, Imaoka Y, Torisu K, et al. A simplified, sensitive immunohistochemical detection system employing signal amplification based on fluorescyl-tyramide/antifluorescein antibody reaction its application to pathologic testing and research. Appl. Immunohistochem. Mol. Morphol. 2008 16 87-93. 

Evaluations of secondary interactive components have been discussed (5-8). In general, the delineation between primary and secondary interactive components have been vague (9). An important advantage of the fluorescein system is that the ligand fills the active site (10-12) which has been conclusively demonstrated by X-ray crystallographic results for the monoclonal antifluorescein antibody (mAb) 4-4-20 (13-15). Thus, interactions with carrier residues associated with the ligand-carrier complex are by necessity outside of the primary interactions. 

DNA probes can be labeled using the modified nucleotide, fluorescein-11-dUTP, and subsequently detected with enhanced chemiluminescence (1) using an antifluorescein antibody conjugated to horseradish peroxidase (2). The use of enhanced chemiluminescence avoids the fading of results associated with some colormetric detection procedures, used with nonradioactive labeling and detection systems. Hard copy results showing excellent resolution are produced. 

Antifluorescein antibody/horseradish peroxidase conjugate. Available from Amersham International (RPN 3022). 

Enzyme conjugate Antifluorescein antibody conjugated to horseradish peroxidase. Conjugate should be stored at 2-8°C. 

Antibodies may be conjugated to enzymes such as alkaline phosphatase or horseradish peroxidase, or to other tags such as fluorescein or biotin (55). The fluorescein is measured by antifluorescein antibodies coupled with an enzyme, and biotin is measured by an avidin-enzyme conjugate, which binds with high affinity to biotin. Michalik et al.(47) used human albumin to tag their second antitoxin, and then used an anti-albumin antibody conjugated to peroxidase for detection of botulism toxins Aand B. They reported a sensitivity for this method of 100 and 300 MLD for toxin types A and B, respectively. 

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