Antennal proteins

Ferkovich S. M., Mayer M. S. and Rutter R. R. (1973b) Sex pheromone of the cabbage looper reactions with antennal proteins in vitro. J. Insect Physiol. 19, 2231-2243. 

Figure 17.2 Purification of M. brassicae male antennal proteins by reverse phase-HPLC. Coomassie blue staining of resulting fractions (T = total antennal extract, 100 antennae). Rectangles indicate the bands submitted to N-terminal sequencing. Each fraction = 200 antennae-equivalent.

Figure 17.3 Binding experiments between tritiated compounds and RP-HPLC-purified antennal proteins. Each fraction corresponds to an aliquot of the purification. One pCi of each pheromonal compound was incubated with 20 pL of each fraction (sample buffer). The films were exposed to the...

Pheromone Deactivation by Antennal Protein of Lepidoptera. Pr. Nauk. Inst. Chem. Org. Fiz. Politech. Wroclaw (22) 955-967 (1981). 

The binding of pheromone molecules by antennal proteins has been observed (340, 341) and transport of a pheromone molecule is considered by some as an integral part of the overall sequence leading to perception (334, 342). In addition, antennae of males and females of Trichoplusia ni contain an enzyme capable of hydrolyzing its pheromone, (2)-7-dodecenyl... 

Riddiford, L. M. Antennal proteins of Saturniid moths—their possible role in olfaction. J. Insect Physiol. 16, 653—660 (1970). 

Using a specific antibody, Shanbhag and collaborators [34] demonstrated that LUSH is expressed in sensilla trichodea of the Drosophila antennae along with two other putative odorant-binding proteins Obp83a (PBPRP-3, OS-F) and Obp83b (OS-E). When antennal sections of the LUSH-deficient mutant were la-... 

Rogers M. E., Krieger J. and Vogt R. G. (2001) Antennal SNMPs (sensory neuron membrane proteins) of Lepidoptera define a unique family of invertebrate CD36-like proteins. J. Neurobiol. 49, 47-61. 

Vogt R. G., Koehne A. C., Dubnau J. T. and Prestwich G. D. (1989) Expression of pheromone binding proteins during antennal development in the gypsy moth Lymantria... 

Klein U. (1987) Sensillum-lymph proteins from antennal olfactory hairs of the moth Antheraea polyphemus (Saturniidae). Insect Biochem. 17, 1193-1204. 

Potential PBPs (OS-E and OS-F) U02545, U02546 identified on the third antennal segment of adult males (McKenna etal. 1994) PBP-related proteins found in different subsets of olfactory sensilla (Pikielny etal. 1994) First insect olfactory receptors (of unknown odorant selectivity) identified on the maxillary palp (Clyne etal. 1999)... 

Only a few proteins from antennal extracts are were able to bind the four compounds tested (Figure 17.3). Fraction 29 included proteins with sequences DAPAA and SEEDK no proteins in this fraction bound compounds. A protein with sequence AKLTT from fractions 30, 31 and 32 bound compounds. The full sequence of the AKLTT protein has been obtained by molecular cloning and identified as a CSP (Jacquin-Joly et al., unpublished, accession number AY026760 see section 17.7). The sequence AKLTT is also found in band 1 of fraction 33 (Figure 17.2), but here this protein fails to bind any compound (Figure 17.3). 

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