Anion-exchange gradient

FIGURE 8.13 Total ion current chromatogram for a UHP-RPLC-MS separation of one anion exchange fraction (fraction numher 6 from the 30-min anion-exchange gradient). 

There workers reported 19 c, values for the anion-exchange gradient elution separation of ovalbumin (M- 44,000), while varying experimental conditions widely column lengths of 7.5 and 15 cm, 30 s to 3 min, and 0.25 F 2 ml/min. A value of Z 8.8 was obtained using the data from Fig. 11 and the approach summarized in Table VI, permitting the prediction of (T, values (as in Table VIII). Good f reement was observed (27), with iLr-1.07 and SD- 0.11. 

FIGURE 14.7 Chromatographic analysis of a nucleotide mixture. Anion exchange gradient method. 

Purine nucleotides were determined by an anion-exchange gradient HPLC method (6). This method separates all major purine nucleotides as well as the ribonucleotide from the deoxyribo-nucleotide form. 

Dried shrimp was ground, defatted with benzene, and then extracted with cold water. The luciferase extracted was purified first by a batch adsorption onto DEAE cellulose (elution with 0.4 M NaCl), followed by gel filtration on a column of Sephadex G-150, anion-exchange chromatography on a column of DEAE-cellulose (gradient elution 0.05-0.5 M NaCl), and gel filtration on a column of Ultrogel AcA 34. The specific activity of the purified luciferase was 1.7 x 1015 photons s 1 mg-1, and the yield in terms of luciferase activity was about 28%. 

Anion-exchange chromatography on a column of TSK DEAE-650 M (EM Science) in 30% methanol. Elution with a linear gradient of NaCl concentration from 0 to 1M. 

It seems that this anionic exchange would be relevant also to explain interactions of trialkyl tin (TAT) compounds (TET, tripropyltin chloride, TBT chloride) with the mitochondria. The current view of this phenomenon is that these compounds, by exploiting the Cl and OH gradient in energized mitochondria, behave as electroneutral OH /Cl exchangers. The crucial point of this new mechanism is that TATs enter the mitochondria as lipophilic cations [RsSn (rV)] and not as electroneutral compounds. The influx is followed by extrusion of the TAT compounds as electroneutral hydroxy compounds RsSnOH. ... 

Figure 3. Typical chromatogram of UV-ahsorhing constituents of a 0.15-ml sample of urine. Conditions 150-cm anion exchange column (1215 fi, Aminex A ZI) bujfer, acetate at pH 4.4 gradient, buffer concentration from 0.015M to 6M flow rate, 10.5 ml/hr, coUimn temperature, 25°C for first 5 hr and 60°C thereafter (34).

Figure 4. Separation of ribonucleoside monophosphofic acids. Conditions 250-cm anion exchange column gradient, 0.01M KHgPO containing HsPOi, (pH 2.6) to 0.15M KHiFO in 30 min column tempera-ture, 70 C detector, UV at 254 nm. 1, cyti-dine-S -monophosphoric acid 2, uridine-5 -monophosphoric acid 3, adenosine-5 -mon-ophosphofic acid 4, inosine-5 -monophosphoric acid 5, 3, 5 -cyclic adenosine mono-phosphoric add 6, guanosine-5 -monophosphoric acid (36).

Figure 6, High pressure liquid chromatogram of creatine kinase isoenzymes. First peak, MM second peak, BB. Conditions 50 cm X 4.8 mm (i.d.) column with yydac porous layer bead anion exchange mobile phase, step gradient Solvent A, 10 mmol/liter Tris buffer, pH 8.3 solvent B, 10 mmol/liter Tris buffer, pH 7.0,0.5 mol KCl flow rate, 2 ml/min detection, collected fractions assayed (45).

Figure 3. Anion-exchange chromatography on DEAE Sepharose of fraction I of the Sephacryl S300 fractionation of a) saponified MHR population A after degradation with RGase and after b) degradation of the non-saponified MHR population A with RGase and RGAEase ---------, uronic acid —, neutral sugars thin line, NaOAc gradient [39],...

The Dionex system uses a Garbo Pac PA-1 anion exchange column and a CarboPac PA-1 Guard. The column was loaded with 25 pi of the RG solution and eluted with a linear gradient of 0 - 0.5 M NaOAc in 0.1 N NaOH during 50 minutes. The flow rate was 1.0 ml/min and the process was monitored using a PE detector. 

Decrease pH by 0.2 units/t, linear gradient (anion exchange) pH varied from 8-2 in 30t,... 

Organic carboxylic acids are commonly found in foods, in the adipate process stream, and as pollutants. Fatty acids are the lipophilic portion of glycerides and a major component of the cell membrane. Phenols are widely used in polymers, as wood preservatives, and as disinfectants. Chloro-phenols such as 4-chlorophenol, two isomeric dichlorophenols, 2,4,6-tri-chlorophenol, three isomeric tetrachlorophenols, and pentachlorophenol were separated on a Dowex (The Dow Chemical Co. Midland, MI) 2-X8 anion exchange resin using an acetic acid-methanol gradient.138... 

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