Aneurine strong

Visual measurement of the thiochrome fluorescence is possible using narrow non-fluorescent tubes, the contents of which are matched in a dark room with an ultra-violet lamp. The test solution and a strong aneurine standard are oxidised and extracted under exactly similar conditions together with a blank from which the ferricyanide has been omitted a measured volume of the test solution is matched against addition of the standard to the blank. The tubes are inclined at an angle of 60°, but they must not be exposed longer than necessary, since the fluorescence is unstable in ultra-violet light. The final comparison is made when the volumes have been equalised. 

Although the fluorimetric method given above is applicable to this preparation the method of Foster and Murfin described in more detail under Strong Compound Tablets of Aneurine can be applied, but for this simpler preparation the following technique may be used ... 

Strong Compound Tablets of Aneurine, B.P.C. Contain 5 mg of aneurine hydrochloride, 2 mg of riboflavine, 20 mg of nicotinamide and 2 mg of pyridoxine hydrochloride. 

Capsules of Vitamins, B.P.C., are assayed for nicotinamide by the microbiological method given above under Nicotinic Acid. Compound Tablets of Aneurine, B.P.C.y and Strong Compound Tablets of Aneurine, B.P.C.y are assayed for nicotinamide by direct hydrolysis as given above under Nicotinamide using the equivalent of 20 and 15 tablets respectively. 

The riboflavine in Compound Tablets of Aneurine and Strong Compound Tablets of Aneurine can be determined by the above method. 

Strong Compound Tablets of Aneurine, B.P.C. These tablets contain 2 mg of pyridoxine hydrochloride together with nicotinamide (20 mg), aneurine hydrochloride (5 mg) and riboflavine (2 mg). The J8.P.C. prescribes a microbiological test for determination of the aneurine and pyridoxine, but the alginic acid separation method of Foster and Murfin is also applicable (see p. 461). 

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