Analysis of the Proteome

Joubert R et al. Two-dimensional gel analysis of the proteome of lager brewing yeasts. Yeast 2000 16 511-522. 

New instrumentation for the analysis of the proteome has been developed including a MALDI hybrid quadrupole time of flight instrument which combines advantages of the mass finger printing and peptide sequencing methods for protein identification (Andersen and Mann 2000). 

Most functional proteomic studies have been performed in cancer cell lines, that is, after exposition to toxicants, RNA inhibition, differentiation agents, viral transfection, and so on. These studies covered several aspects of mutagenesis, tumor promotion, and progression. In the recent years, it has been shown that repeated analysis of the proteome at different tumor stages also deliver distinct patterns and thus a functional picture of disease progression at the molecular level. 

Recent proteomic analysis indicated that the cilia of mammalian photoreceptor cells are significantly more complex than other eukaryotic cilium (Liu et al. 2006). Over 1200 different polypeptides have been identified by the quantitative analysis of the proteome of photoreceptor outer segment compared with the proteome of the axoneme/ciliary fraction of mouse photoreceptor cells. This data set contains all previously identified protein components of the photoreceptor cilium (e.g. Schmitt and Wolfrum 2001). An understanding how the identified proteins function in their native environment of the diverse compartments of the photoreceptor cilia will require increased knowledge of their molecular interaction and networking. Insights into the organization and composition of diverse protein complexes may also provide novel information on how functional modules of the cell, recently proposed by Hofmann et al. 2006, are connected. 

De Nobel, H., Lawrie, L., Brul, S., et al. 2001. Parallel and comparative analysis of the proteome and transcriptome of sorbic acid stressed Saccharomyces cerevisiae. Yeast 18 1413-1428. 

Beranova-Giorgiarmi, S., F. Giorgianni, et at, 2002, Analysis of the proteome in the human pituitary. Proteomics 2 534-42. 

Zhan, X. and Desiderio, D.M., Comparative proteomics analysis of human pituitary adenomas current status and future perspectives. Mass Spectrom. Rev., 24, 783-813 (2005). Beranova-Giorgianni, S., Giorgianni, F. and Desiderio, D.M., Analysis of the proteome in the human pituitary. Proteomics, 2, 534-542 (2002). 

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