Analysis lectin

Tomato PGIP with respect to its molecular weight falls in between pear PGIP and bean PGIP [22]. The presence of G-linked glycosyl side chains carmot be ruled out, but initial MALDI-TOF and lectin analysis experiments indicate that for the bean PGIP, all carbohydrate is iV-linked. 

Bundy, J. Fenselau, C. Lectin-based affinity capture for MALDI-MS analysis of bacteria. Anal. Chem. 1999, 71,1460-1463. 

Kelly D. and King T.P. (1991). The influence of lactation products on the temporal expression of histo-blood group antigens in the intestines of suckling pigs lectin histochemical and immunohistochemical analysis . Histochem J, 23, 55-60. 

T. K. Dam, R. Roy, D. Page, and C. F. Brewer, Negative cooperativity associated with binding of multivalent carbohydrates to lectins. Thermodynamic analysis of the multivalency effect Biochemistry, 41 (2002) 1351-1358. 

Interestingly, one of the conformers obtained from data analysis corresponds to the bound conformation of the same chain in a biantennary oligosaccharide-lectin complex from X-ray diffraction. 

Eiljeblad M., Eundblad A., and Pahlsson P. (2002), Analysis of glycoproteins in cell culture supernatants using a lectin immunosensor technique, Biosens. Bioelectron. 17, 883-891. 

A., Kominami, J., Tatsumi, N., Osaka, Y, Maruyama, S., Ueda, T., Hirabayashi, J. Comprehensive interaction analysis between lectins and PA-oligosaccharides by an automated frontal afhnity chromatography (FAC) system. Glycobiology 2004, 14, 1189-1189. 

Andre, S. Pei, Z. Siebert, H.-C. Ramstrom, O. Cabins, H.-J. Glycosyl-disulfides from dynamic combinatorial libraries as O-glycoside mimetics for plant and endogenous lectins Their reactivities in solid-phase and cell assays and conformational analysis by molecular dynamics simulations. Bioorg. Med. Chem. 2006,14, 6314-6326. 

---