Amplicon-Based Vectors

Vector Packaging Capacity Titer Integration Expression Tropism Transduction Efficiency 

Adeno- associated virus 5kb 109 Yes ( ) Transient Broad except for hematopoietic cells High 

HSV-1 40-150 kb 108 No Transient except neurons Strong for neurons High 

Adenovirus dsDNA Excellent transduction of most tissues Potent inflammatory response against the virus 

Adeno-associated ssDNA No toxicity. No Limited packaging 

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Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

Sabbioni S, Callegari E, Manservigi M, Argnani R, et al. 2007. Use of herpes simplex virus type 1-based amplicon vector for delivery of small interfering RNA. Gene Ther. 14 459 164. 

The development of vectors based on HSV-1 has produced two different viral vector systems recombinant HSV-1 vectors and HSV-1 amplicons. 

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