Amniotic fluid aspiration

Birth asphyxia Hypothermia Meconium or amniotic fluid aspiration Necrotizing enterocolitis Respiratory distress syndrome Shock Obstetrics Abortion... 

The rat is placed in a dorsal position on an undersheet and the fur is moistened with ethanol/water. The abdominal cavity is opened and the abdominal wall folded back. The uterus is exposed and the amniotic fluid is withdrawn by puncturing the amniotic sac with a syringe. The uterus is then placed on a plastic film and opened. The placentas with the fetuses are detached from the uterus. The placentas are then detached from the fetuses. After removing the fetal membranes the fetuses are removed and immediately killed under CO2 atmosphere. The amniotic fluid is aspirated with a disposable syringe. 

At specific intervals, amniotic fluid was aspirated for column chromatography. In addition, total nitrogen, -amino nitrogen, urea, uric acid, creatinine as well as certain enzymes were also determined. 

Intrauterine infusion (intra-amniotic or extra-amniotic) has been reported to be associated with fewer gastrointestinal symptoms and less fever than parenteral or intra-vaginal administration (122). In intra-amniotic use, the puncture must be guided by ultrasonography, and before injection a control aspiration of some amnio tic fluid is required in order to avoid intrauterine or intravascular injection. Uterine rupture has been described with intra-amniotic treatment. 

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