6-Aminoquinolyl-N-hydroxysuccinimidyl

Emission and excitation wavelengths are solute- and solvent dependent. A number of the excitation wavelengths correspond to the laser lines (325, 442, and 488 nm) and do not correspond to the actual excitation maxima. In addition, the excitation wavelength is frequently selected to avoid the Raman band and may not correspond to the actual emission maximum. c 3-(4-Carboxybenzoyl)-2-quinolinecarboxaldehyde, available from Molecular Probes. d 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate, available from Waters Chromatography. 

Ahmed, N., Argirov, O.K., Minhas, H.S., Cordeiro, C.A., and Thomalley, P.J. 2002. Assay of advanced glycation endproducts (AGEs) surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to Nepsilon-carboxymethyl-lysine- and Nepsilon-(l-carboxyethyl)lysine-modified albumin. Biochem J 364 1-14. 

Amino Acid Analysis of Unusual and Complex Samples Based on 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization... 

The analysis of cell culture media and supernatants, as well as non-standard protein hydrolysates such as collagens and glycoproteins, has created the demand for techniques that accurately quantify additional amino acids not normally found in hydrolyzed samples, Methods of amino acid analysis (AAA) based on precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) have previously been shown to quantify hydrolyzed samples with a high degree of accuracy (1,2). The AQC-based method has also been shown to derivatize effectively in the presence of salts and lipids (3). Taking into account the above strengths, the excellent stability of the derivatives, and the unique fluorescence properties that allow for direct injection of the reaction mixture without cleanup, the AQC methodology represents an ideal choice for the analysis of complex samples. 

Abbreviations used AAA, amino acid analysis ABRF, Association of Biomolecular Resource Facilities AQC, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate FMOC, N-(9-fluorenylmethoxycarbonyl) OPA, o-phthaldialdehyde PITC, phenylisothiocyanate tpis, triosephosphate isomerase. 

M. Pawlowska, S. Chen and D.W. Armstrong, Enantiomeric Separation of Fluorescent 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate, Tagged Amino Acids, J. Chromatogr., 641(1993)... 

AQC 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate, a fluorescent tagging agent reaction mixture of AQC and amino acid was used without purifying the derivatives. 

Oreiro-Garcia MT, Vazquez-Illanes MD, Sierra-Paredes G, Sierra-Marcuno G. Analysis of neuroactive amino acids from microdialysate samples by fluorescence detection using a modification of the 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate method. Biomed Chromatogr BMC 2005 19 720-4. 

Cohen, S. and D. Michaud, 1993. Synthesis of a fluorescent derivatizing reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and its application for file analysis of hydrolysate Amino Acids via High-Performance Liquid Chromatography. Anal. Biochem. 211,279-287. 

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