6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate , derivatization

Amino Acid Analysis of Unusual and Complex Samples Based on 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization... 

Ahmed, N., Argirov, O.K., Minhas, H.S., Cordeiro, C.A., and Thomalley, P.J. 2002. Assay of advanced glycation endproducts (AGEs) surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to Nepsilon-carboxymethyl-lysine- and Nepsilon-(l-carboxyethyl)lysine-modified albumin. Biochem J 364 1-14. 

The analysis of cell culture media and supernatants, as well as non-standard protein hydrolysates such as collagens and glycoproteins, has created the demand for techniques that accurately quantify additional amino acids not normally found in hydrolyzed samples, Methods of amino acid analysis (AAA) based on precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) have previously been shown to quantify hydrolyzed samples with a high degree of accuracy (1,2). The AQC-based method has also been shown to derivatize effectively in the presence of salts and lipids (3). Taking into account the above strengths, the excellent stability of the derivatives, and the unique fluorescence properties that allow for direct injection of the reaction mixture without cleanup, the AQC methodology represents an ideal choice for the analysis of complex samples. 

Cohen, S. and D. Michaud, 1993. Synthesis of a fluorescent derivatizing reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and its application for file analysis of hydrolysate Amino Acids via High-Performance Liquid Chromatography. Anal. Biochem. 211,279-287. 

---