N-Hydroxysuccinimidyl

OTcp 2,4,5-trichlorophenyl ester ONSu N-hydroxysuccinImidyl ester N azide... 

HSAB (N-hydroxysuccinimidyl-4-azidobenzoate) is a heterobifunctional reagent containing an amine-reactive NHS ester on one end and a photoreactive phenyl azide group on the other end... 

A modification of Zalipsky s method by Miron and Wilchek (1993) simplifies the creation of the SC-activated species. Instead of using highly toxic phosgene to form a chloroformate intermediate and then reacting with NHS, the new procedure utilizes either N-hydroxysuccinimidyl chloroformate or N,N -disuccinimidyl carbonate (DSC Chapter 4, Section 1.7) to produce the SC-PEG in one step (Figure 25.6). Since both activation reagents are commercially available, creating an amine-reactive PEG derivative has never been easier. 

Figure 25.6 An alternative route to an SC derivative of mPEG can be accomplished by the reaction of the terminal hydroxyl group of the polymer with either N,N -disuccinimidyl carbonate or N-hydroxysuccinimidyl chloroformate.

Fig. 11 Examples of heterobifunctional crosslinkers a N-hydroxysuccinimidyl-4-azido-benzoic acid (HSAB) and b Lomant s reagent...

Emission and excitation wavelengths are solute- and solvent dependent. A number of the excitation wavelengths correspond to the laser lines (325, 442, and 488 nm) and do not correspond to the actual excitation maxima. In addition, the excitation wavelength is frequently selected to avoid the Raman band and may not correspond to the actual emission maximum. c 3-(4-Carboxybenzoyl)-2-quinolinecarboxaldehyde, available from Molecular Probes. d 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate, available from Waters Chromatography. 

Ahmed, N., Argirov, O.K., Minhas, H.S., Cordeiro, C.A., and Thomalley, P.J. 2002. Assay of advanced glycation endproducts (AGEs) surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to Nepsilon-carboxymethyl-lysine- and Nepsilon-(l-carboxyethyl)lysine-modified albumin. Biochem J 364 1-14. 

The N-hydroxysuccinimidyl hydrazinonicotinate (S-HYNIC) moiety forms a very stable Tc complex and provides excellent images. HYNIC has been successfully coupled to peptides and antibodies for imaging thrombosis and sites of infection. HYNIC also has been used for attachment to Annexin for in vivo imaging of apoptosis at the molecular level. 9.2o... 

A solution of 100 p,g IgG in 500 (jlI 0.2 M NaHCO3/0.5 M NaCl (pH 8) was added to n-hydroxysuccinimidyl (NHS)-activated 6-aminohexanoic acid-coupled sepharose (Sigma, St Louis, USA), and allowed to bind for 60 min at 20 C before transferring onto a microcapillary (MoBiTec, Gottingen, Germany). Columns were washed alternately with blocking (ethanolamine/NaCl) and washing buffer (NaAc/NaCl). 

Amino Acid Analysis of Unusual and Complex Samples Based on 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization... 

The analysis of cell culture media and supernatants, as well as non-standard protein hydrolysates such as collagens and glycoproteins, has created the demand for techniques that accurately quantify additional amino acids not normally found in hydrolyzed samples, Methods of amino acid analysis (AAA) based on precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) have previously been shown to quantify hydrolyzed samples with a high degree of accuracy (1,2). The AQC-based method has also been shown to derivatize effectively in the presence of salts and lipids (3). Taking into account the above strengths, the excellent stability of the derivatives, and the unique fluorescence properties that allow for direct injection of the reaction mixture without cleanup, the AQC methodology represents an ideal choice for the analysis of complex samples. 

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