Amino acid code

Fig. 3. The hierarchy of protein stmctures (a) primary stmcture (see Table 1 for amino acid code) (b) secondary stmcture (c) tertiary stmcture and (d)...

Prostanoids. Figure 2 EP3 receptor sequence of three mouse EP3 receptor splice variants differing only in their intracellular carboxyl termini. The predicted amino acid sequences of each splice variant are represented by the one letter amino acid code. The common region is comprised of two exons, which are spliced to three possible C-terminal tails. The carboxyl variable tails are designated alpha, beta, and gamma,each encoded by distinct exons. 

Gu, S Du, Y Chen, J., Liu, Z Bradbury, E.M., Hu, C.A., Chen, X. (2004). Large-scale quantitative proteomic study of PUMA-induced apoptosis using two-dimensional liquid chromatography—mass spectrometry coupled with amino acid-coded mass tagging. J. Proteome Res. 3, 1191 1200. 

No Amino Acid Code Subl Sub2 Sub3 Sub4 Sub5 Sub6 Sub7 Sub8... 

Site-directed mutants of peroxidase enzymes are represented using one-letter amino acid codes in the style X residue number X peroxidase, which indicates that amino acid X has been substituted by amino acid X. ... 

The location of the amino acid residues is shown in Fig. 4. Single letter amino acid codes are used for the mutants. 

Table 8.13. Amino acids code and molecular mass...

The specific ribonucleotide sequence in the mRNA forms a code that determines the order in which the different amino acid residues have to be joined. The four bases, i.e.. A, C, G and U have been shown to act in the form of triplets. Since a particular amino acid codes a specific amino acid, therefore, these triplets are called codons. Since there are four bases, therefore, 4 = 64 triplets or codons are possible. 

Signals are identified using their one-letter amino acid code. 

Schneider, K. et al.. The substrate specificity-determining amino acid code of 4-coumarate CoA ligase. Proc. Natl Acad. Scl USA, 100, 8601, 2003. 

Consolidation of the results from many experiments permitted the assignment of 61 of the 64 possible codons. The other three were identified as termination codons, in part because they disrupted amino acid coding patterns when they occurred in a synthetic RNA polymer (Fig. 27-6). Meanings for all the triplet codons (tabulated in Fig. 27-7) were established by 1966 and have been verified in many different ways. The cracking of the genetic code is regarded as one of the most important scientific discoveries of the twentieth century. 

FIGURE 27-7 "Dictionary" of amino acid code words in mRNAs. 

Elongation continues until the ribosome adds the last amino acid coded by the mRNA. Termination, the fourth stage of polypeptide synthesis, is signaled by the... 

Was this youT answeT A codon is a triplet of mRNA nucleotides holding instructions for the building of a polypeptide strand, and so this sequence contains seven codons. The first andlast are start/stop instructions, however, and so the number of amino acids coded for is only five. The sequence is Leu-Lys-Ser-Gl n-Al a. 

Each amino acid code consists of a three-base sequence. For example, the DNA sequence CGA codes for the amino acid alanine.6 This sequence in DNA codes for the m-RNA sequence GCU. A three-base pair in m-RNA that codes for an amino acid is called a codon. An anticodon is the three-base sequence in t-RNA that matches a codon in m-RNA. 

Note The kmIK values were determined by plotting the initial reaction rates versus substrate concentrations using the equation of v = (fct /A )[E][S, where [E] and [S] are the enzyme and substrate concentrations, respectively. The enzyme concentration was used at 0.1 pM for 3C and 0.4 pM for 2A protease. The reactions were performed at 30°C in a 200-pl reaction mixture containing 25 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM EDTA, and 250 pM pNA peptide substrate and monitored by absorbance at 405 nm. Amino acid sequences of the pNA peptides are given in single-letter amino acid code. NC, no cleavage ND, not determined. 

Note The protease reactions were performed under the conditions described in the text and monitored using a Perkin-Elmer LS50B fluorometer. The kcJKm values were determined as described in Table 1 legend. Peptide sequences are given in single-letter amino acid code. NC, no cleavage ND, not detected. 

Code Amino acid Code Amino acid Code Amino acid Code Amino acid... 

The NH2-terminal amino acid sequence of the activated wild-type tyrosinase precipitated with 5% (w/v) trichloroacetic acid was SDKIHLTDD (single-letter amino acid codes), which is known to be the NH2-terminal sequence of thioredoxin including the 10 residues from the Ser2 to Aspll. Although the initiating methinine residue was encoded in the construct, it seems to have disappeared after cleavage of the protein. 

Vrtth prefer 100 yM) OWfnigM, Specificity element variations in single teller amino acid codes... 

EXON The portion of a gene that contains the amino acid coding sequences and that remains represented in mature mRNA after mRNA precursors have been spliced to remove internal noncoding regions (i.e., introns). 

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