Amberlite-IRC

Amberlite IRC-50 3.5 1.25 Methacrylic acid-DVB. Selectivity adsorbs organic gases such as antibiotics, alkaloids, peptides, and amino acids. Use pH >5. 

Myoglobin (from sperm whale muscle). [9047-17-0] M, -17,000. Purified by CM-cellulose chromatography and Sephadex G-50 followed by chromatography on Amberlite IRC-50 Type III or BioRex 70 (<400mesh). The crystalline product as a paste in saturated (NH4)2S04 at pH 6.5-7.0 may be stored at 4° for at least 4 years unchanged, but must not be kept in a freezer. [Anres and Atassi Biochemistry 12 942 1980, Edmundson Biochem Prep 12 41 1968.]... 

Filtered broth was passed at 2.5 ml/min through a resin column (2.5 cm diameter, 28 cm length) packed with 150 ml of ion exchange resin Amberlite IRC-50 sodium type (Rohm and Haas Co., U.S.A.). The column was washed with water, eluted with 0.5 N HCI at a flow rate 1.3 ml/min. The eluates were fractionated each 10 ml and tuberactinomycin-N activity was found at fractions No. 45-63 obsarved by ultraviolet absorption method and bioassay. 

In contrast to the above resins, the chelating resin Amberlite IRC-718 is based upon a macroreticular matrix. It is claimed to exhibit superior physical durability and adsorption kinetics when compared to chelating resins derived from gel polymers and should also be superior for use in non-aqueous solvent systems. 

Waksman et al, in 1949, first reported the production of neomycin by fermentation of a culture of S. faUcid-Lcut. (3535). The same organism subsequently formed the basis of an industrial fermentation process for the biosynthesis of neomycin287,288. isolation of the antibiotic from the fermentation media is accomplished by use of ion-exchange resins, such as Amberlite IRC 50 25,250,251. 

The isolation of neamine on Amberlite IRC 50(Na form) following the acid hydrolysis of neomycin has been described- -9. Neamine is eluted from the ion-exchange column with hydrochloric acid. This process had been patented 9 as a means of manufacturing neamine. 

Ovaries (100 g) of the pufferfish Fugu vermicularis porphgreus are extracted with 1% acetic acid in methanol. The extract is concentrated in vacuo and defatted by shaking with chloroform. The defatted extract is treated with activated charcoal. The toxin adsorbed is eluted with 1% acetic acid in 20% ethanol. The eluate is evaporated in vacuo to dryness. The residue is dissolved in a small a-mount of water and adjusted to pH 6 with 1 N NaOH. The toxic solution is applied to an Amberlite IRC-50 column (NH, 2.5 x 45 cm) and developed with 2 L of water, and then 1 L each of 1 and 10% acetic acid. The toxic fractions are freeze-dried, dissolved in 1 mL of water and analyzed by HPLC. 

Carboxylic acid-type Weak acid cation exchangers Amberlite IRC-50 Bio-Rex 70 Che lex 100 Duolite 436 Permutit C Permutits H and H-70 (Rohm and Haas, USA) (Bio-Rad, USA) (Bio-Rad, USA) (Dia-Prosim Ltd) (Permutit AG, Germany) (Permutit Co, USA)... 

The aldehyde 5 (2.33 g, 7.5 mmol) in dry methanol (100 mL) containing NaOMe (1 g) was kept at 25°C for 30 min. The solution was neutralized by stirring with a carboxylic acid-based cation-exchange resin (e.g., Amberlite IRC-50, H+), the mixture was filtered, and the... 

Extraction with MeOH, three elutions through pH 7-buffered Amberlite IRC-50 Plus cation-exchange column, two elutions through pH 4.25-buffcrcd column of the same material, elution with MeOH-HCl, evaporation at 65°C, addition of saturated NaHCO,-McOH, derivatization with DNS. 

Dansylation is often used for the determination of free and N-terminal amino acids. Dansyl chloride (5-dimethylaminonaphthalene-l-sulfonyl chloride, DNS-C1) reacts with the amino substituent of amino acids to form highly fluorescent derivatives [75,76]. The method is particularly useful for the analysis of trace components due to the high sensitivity of the products. The derivatives are usually separated by TLC on various types of layers. Separations of DNS-amino acids by flat-bed techniques have been reviewed [77]. Separations by column chromatography have been examined on polyamide [78] and Amberlite IRC-SO [79]. Although many variations of the dansylation reaction with amino acids have been reported, the one described below [77] appears to be the most common. 

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