Ergot alkaloids, analysis

Poisoning by ergot still occurs occasionally in countries where rye is extensively used as a food grain or where ergotised grain is liable to be fed to cattle and methods for its detection have been devised, but interest in the analysis of ergot centres chiefly on the estimation of the active alkaloids in the crude drug or its preparations. The methods used may... 

In addition to the effects noted above, the ergot alkaloids produce a variety of other central nervous system and peripheral effects. Detailed structure-activity analysis and appropriate semisynthetic modifications have yielded a large number of agents of interest. 

Gunatilaka AA. Natural products from plant-associated microorganisms distribution, structural diversity, bioactivity, and imph-cations of their occurrence. J. Nat. Prod. 2006 69 509-526. Porter JK. Ergot alkaloids and alkaloids from other endophytes, responsible for causing toxic syndrome in cattle after eating contaminated grass. Prikl. Biokhim. Mikrobiol. 1993 29 51-55. Porter JK. Analysis of endophyte toxins fescue and other grasses toxic to livestock. J. Anim. Sci. 1995 73 871-880. 

Reversed-phase HPLC has been widely used for the analysis of both LSD and ergot alkaloids. 

The first reversed-phase separation of ergot alkaloids was reported by Jane and Wheals in connection with the analysis of LSD. Pellicular beads with chemically bonded octadecyl groups were used in combination with methanol - 0.1% aqueous ammonium carbonate (3 2) as mobile phase However, the mobile phase proposed by Vivilecchia et al.6 for the separation of the dihydro-ergotoxine alkaloids on octadecyl columns (acetonitrile - aqueous ammonium carbonate) has been... 

Jane separated a wide range of drugs of abuse on microparticulate silica gel with polar mobile phases containing ammonium nitrate solutions. LSD could be analyzed with methanol -0.2 M ammonium nitrate solution (3 2)(Fig.9.6). The system has been successfully applied to the analysis of ljd13 15 16 28 45 46, but for the analysis of ergot alkaloids the above mentioned reversed-phase system gave better results3 (Table 9.5). 

For the LC-MS analysis of clavine alkaloids in ergot fermentation broth, Eckers et al. separated the alkaloids on silica gel with the mobile phase dichloromethane - methanol - concentrated ammonia (95 5 0.1)(Fig.9.10). 

Fluorescence detection has found wide application in the analysis of ergot alkaloids 36,50 ScQtt and Lawrence50 found that for the fluorimetric detector used in their investi-... 

Eckers et al. reported the analysis of clavine alkaloids 1n ergot fermentation broth... 

HPLC ANALYSIS ERGOT ALKALOIDS IN AND FROM FUNGI... 

The present invention relates to the manufacture, separation and preparation of ergot-alkaloids in state of high purity, by subjecting mixtures of the very sensitive ergot-alkaloids to chromatographic adsorption analysis. 

Agurell, S., Ramstad, E. Analysis of Clavine Alkaloids of Pennisetum Ergot Lloydia (1962) 25 67-77... 

Of relevance from a practical point of view are publications dealing with the t.l.c.-quantitative analysis of twelve Rauwolfia bases, " t.l.c.-colour reactions for analysis of 38 indole alkaloids from some Vinca species, the quantitative determination of Cinchona quinoline alkaloids by t.l.c.-differential spectrophotometry, and the high-speed liquid-liquid chromatography of ergot alkaloids " and Mitragyna alkaloids. ... 

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