Ergot alkaloids analysis techniques

The combination of classical and molecular genetic techniques opens interesting new perspectives of the analysis of ergot alkaloid biosynthesis in Claviceps and for the development of strain improvement programs. The available molecular techniques should allow in the near future the cloning of important genes of the... 

The variety of applications of ion-pair chromatography illustrates the versatility of this technique in separation science. Early applications utilized a normal-phase solid support and explored the resolution of amines and alkyl ammonium ions with an anionic counter ion such as picrate or naphthalene 2-sulfonate (41, 42, 89, 90, 219). These results prompted investigations into the suitability of ion-pair chromatography as a technique for use in pharmaceutical analysis such as the determination of ergot alkaloids (333). 

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