Alkaline phosphatase TOPICAL

A more critical approach to histochemical problems was heralded by the publication of Lison s Histochemie Animale in 1936 (55). Some of Lison s original studies dealt with the meaning of metachromasia, the effects of fixatives on glycogen, and other topics of carbohydrate histochemistry. Other milestones in histochemistry were the alkaline phosphatase method of Gomori (57) and the periodic acid - Schiff method for carbohydrates developed by McManus (55), Lillie 59), and Hotchkiss (50). The technical performance and fruitful results of these two methods did much to show the possibilities of histochemistry in histology and pathology. 

The enzymes present in Aloe vera include aliiase, alkaline phosphatase, amylase, bradykinase, carboxypeptidase, catalase, cellulase, lipase, and peroxidise. Bradykinase prevents inflammation while applied topically on the skin while the others partieipate in metabolism of fats and sugars (Malik Itrat et al., 2013 Javed and Rahman, 2014). 

Scaffolds made of PCL and HAp were a topic of investigations of Causa and coworkers [164]. They found that the mechanical properties of the composites are close to those of human bone only after the addition of 20 vol% of HAp. In particular, the elastic modulus is within the range of values for human cortical bone. Moreover, with the use of primary human osteoblasts, a high proUferation rate and a moderate increase of alkaline phosphatase activity were found, mainly on the surface of PCL-based composites with 13 and 20 vol% of HAp, though at the last time point (4 weeks) all the HAp-added polymers were covered by confluent layers of cells. It was concluded that the structure of a scaffold along with its surface physicochemical characteristics affect cell behaviour, but, on the other hand, mechanical properties are also crucial for implant performance. 

---